脂多糖、多巴胺与蛋白激酶抑制剂联合作用对凡纳滨对虾血细胞吞噬率、胞吐酚氧化酶活力的影响  

Haemocyte phagocytosis,exocytosis and their signal transduction in white shrimp (Litopenaeus vannamei) induced by lipopolysaccharide and dopamine

作　　者：潘鲁青[1] 谢鹏[1] 岳峰[1] 孙晓华[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《水产学报》2010年第5期726-732,共7页Journal of Fisheries of China

基　　金：教育部新世纪优秀人才支持计划(NCET-06-0597);高等学校创新引智计划(B08049)

摘　　要：研究了脂多糖(LPS)、多巴胺(DA)对凡纳滨对虾血细胞吞噬、胞吐及信号通路的影响。结果表明,LPS、DA对凡纳滨对虾血细胞数量、吞噬率和胞吐酚氧化酶活力影响显著(P<0.05),LPS、DA与血细胞孵育30min后,高浓度(1～10mg/L或μmol/L)处理组对虾血细胞数量均随作用浓度增大而明显下降,胞吐酚氧化酶活力则显著升高,LPS处理组对虾血细胞吞噬率显著升高,而DA处理组对虾血细胞吞噬率则表现出明显的下降趋势。同时在LPS作用下,分别加入蛋白激酶C(PKC)、酪氨酸蛋白激酶(TPK)抑制剂chelerythrine、genistein后,凡纳滨对虾血细胞吞噬和胞吐作用均受到明显的抑制作用,抑制剂对吞噬的抑制效果为genistein>chelerythrine,对胞吐的抑制效果则为chelerythrine>genistein;在DA作用下,加入蛋白激酶A(PKA)抑制剂H-89后,血细胞吞噬作用得到增强,但对胞吐作用无明显影响,chelerythrine、genistein均抑制对虾血细胞的胞吐作用,抑制剂的作用效果为chelerythrine>genistein,两种抑制剂对吞噬均无显著影响。Effects of bacterial lipopolysaccharide （ LPS） and dopamine （ DA） on phagocytic activity, exocytosis and signal transduction of haemocytes in white shrimp （Litopenaeus vannamei） were studied. The haemocytes treated with LPS （1 - 10 mg/L） and dopamine （1 - 10 μmol/L） in vitro showed conspicuous dose-dependent decrease in cell count,and increase in extracellular phenoloxidase （PO） activity. Phagocytic activity of haemocytes was promoted by LPS and inhibited by dopamine. Meanwhile,the addition of chelerythrine （a PKA inhibitor） and genistein （a TPK inhibitor） to haemocytes can inhibit phagocytosis and exocytosis induced by LPS,and the inhibitory efficiencies on phagocytosis and exocytosis respectively were： genistein chelerythrine,chelerythrine genistein. Dopamine-induced cell phagocytosis was strenghtend by H-89 （a PKA inhibitor）,its exocytosis was inhibited by chelerythrine and genistein,and the efficiency was chelerythrine genistein,and the two inhibitors had no impact on dopamine-induced phagocytosis.

关 键 词：凡纳滨对虾 血细胞 胞吐 吞噬 脂多糖 多巴胺 信号通路 

分 类 号：S942.5[农业科学—水产养殖]