柱前衍生-反相高效液相色谱法测定人参中人参二醇  

RP-HPLC Determination of Panaxadiol in Ginseng with Pre-column Derivatization

作　　者：石李梁[1] 钦维民[1] 朱智甲[1] 林苗[1] 王改香[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《理化检验（化学分册）》2010年第5期482-484,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用苯甲酰氯为柱前衍生化试剂,固定相为Hypersil ODS2（4.60 mm×150 mm,5μm）,流动相为乙腈-水（40＋60）混合溶液,以230 nm作为检测波长,采用峰面积外标法定量,提出了测定人参中人参二醇的柱前衍生-反相高效液相色谱方法。在0.01-0.10 g.L-1范围内,人参二醇衍生物的色谱峰面积与其质量浓度呈线性关系,检出限（3S/N）为0.07 mg.L-1,取0.06 g.L-1人参二醇衍生化标准溶液重复进样6次,做精密度试验,得相对标准偏差为1.61%。A new method of RP-HPLC with precolumn derivatization was proposed for the determination of panaxadiol in ginseng.Benzoyl chloride was used as derivatizing agent.The Hypersil ODS2 column（4.60 mm×150 mm,5 μm） was used as stationary phase,and a mixed solvent of acetonitrile and water（40＋60） was used as mobile phase.UV-detection at the wavelength of 230 nm and the external standard method were adopted in the determination.Linear relationship between values of peak area and mass concentration of panaxadiol（in form of its derivatized product） was obtained in the range of 0.01-0.10 g·L-1.Detection limit（3S/N） found for this method was 0.07 mg·L-1.Precision of the method was tested with 0.06 g·L-1 of the standard panaxadiol solution for 6 replicate determination including the precolumn derivatization process,value of RSD calculated from data of peak are was 1.61%.

关 键 词：反相高效液相色谱法 柱前衍生 人参 人参二醇 

分 类 号：O657.7[理学—分析化学]