反相高效液相色谱法同时测定功能饮料中六种B族维生素  

Simultaneous Determination of Six B-Group Vitamins in Functional Drinks by HPLC

作　　者：徐烨[1] 顾鑫荣[1] 王瑞菲[1] 曹轶男[1] 

机构地区：[1]东北大学理学院,沈阳110004

出　　处：《理化检验（化学分册）》2010年第5期497-499,502,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了反相高效液相色谱法同时测定功能饮料中6种B族维生素,维生素B1、维生素B2、维生素B6、烟酸、烟酰胺和泛酸的方法。对色谱分离条件做了试验并予以优化。以KromasilC18色谱柱（200 mm×4.6 mm,5μm）为分离柱,用甲醇（A）和0.3%（体积分数）三乙胺溶液（B）（磷酸调节pH至3.5）以不同比例混合进行梯度淋洗,选择在200 nm波长处测定泛酸,在270 nm波长处测定其他5种B族维生素。6种B族维生素的质量浓度在0.5-100 mg.L-1范围内与其峰面积呈线性关系。标准加入回收率在97.8%-106.2%之间,相对标准偏差（n=5）在0.5%-2.3%之间。RP-HPLC was applied to the simultaneous determinations of six B-group vitamins including vitamin B1,vitamin B2,vitamin B6,niacin,nicotinamide and pantothenic acid in functional drinks.The conditions of separation by liquid chromatography were tested and optimized.The Kromasil C18 column（200 mm×4.6 mm,5 μm） was used as stationary phase,and mixtures of methanol（A） and triethylamine solution（φ=0.3%,adjusted to pH 3.5 with H3PO4）（B） in different ratios were used as mobile phase in the gradient elution.UV-detections were used at the wavelength of 200 nm for determination of pantothenic acid and at 270 nm for the other 5 vitamin B′s.Linear relationships between the values of peak area and mass concentration of the six B-group vitamins were obtained in the range of 0.5~100 mg·L-1.The values of recovery found by standard addition method were in the range of 97.8%-106.2%,and values of RSD′s（n=5） were in the range of 0.5%-2.3%.

关 键 词：反相高效液相色谱 功能饮料 B族维生素 

分 类 号：O657.62[理学—分析化学]