木素小分子愈创木基型松柏醇漆酶生物改性的光谱分析  

Spectra Analysis of Lignin Small Molecular Guaiacyl Coniferyl Alcohol Biological Modification Treated by Laccase

作　　者：刘海棠[1] 裴继诚[1] 胡惠仁[1] 裴雅[1] 

机构地区：[1]天津科技大学天津市制浆造纸重点实验室,天津300457

出　　处：《光谱学与光谱分析》2010年第6期1469-1473,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30771694);天津市科技计划发展项目(06TXTJJC14302)资助

摘　　要：从分子模型物的角度入手,通过气相色谱-质谱联用(GC-MS)、凝胶渗透色谱(GPC)、红外光谱和颗粒电荷等测试手段,综合考察了漆酶对针叶木和阔叶木中的愈创木基型木素-松柏醇生物改性的机理。松柏醇经漆酶处理后,GC-MS检测不到松柏醇单体的存在,推测松柏醇参与了化学反应,结构发生变化。GPC分析证明松柏醇漆酶处理后分子量变大,松柏醇发生了聚合。红外光谱确定了松柏醇漆酶处理过程中反应位点,主要是酚羟基、苯环上的甲氧基、苯环侧链上的双键,β碳、γ碳也有可能参与了反应。颗粒电荷测定仪(PCD)测得阳离子需求量下降了88.38%。The enzymatic modification mechanism of lignin small molecular lignin guaiacyl coniferyl alcohol existing in softwood and hardwood treated by laccase was studied.Gas chromatography-mass spectrometry （GC-MS）,gel permeation chromatography （GPC）,FTIR spectrum and PCD（particle charge detector） etc were used for the measurement.GC-MS could not detect the coniferyl alcohol monomer after treated by laccase,so it was speculated that coniferyl alcohol participated in the reaction totally,and the structure of coniferyl alcohol was changed.GPC demonstrated that the molecular weight of coniferyl alcohol increased when it was treated by laccase,and coniferyl alcohol was polymerized.FTIR spectrum determined the reaction point of coniferyl alcohol when treated by laccase,and it is mainly phenolic hydroxyl,aromatic ring side chain substituent such as methoxy,double bound in side chain,β-carbonyl groups,and γ-carbonyl groups.PCD gave a result that the cationic demandv（CD） decreased by 88.38%.

关 键 词：松柏醇 气相色谱-质谱联用 凝胶渗透色谱 红外光谱 漆酶 

分 类 号：O657[理学—分析化学] O636.2[理学—化学]