特发性多发性肌炎外周血淋巴细胞共刺激分子表达的研究  

Study on the expression of costimulatory molecules in peripheral blood lymphocytes in idiopathic polymyositis

作　　者：骆文静[1] 蒲传强[1] 

机构地区：[1]解放军总医院神经内科,100853

出　　处：《神经疾病与精神卫生》2010年第2期115-118,共4页Journal of Neuroscience and Mental Health

摘　　要：目的观察特发性多发性肌炎患者外周血淋巴细胞共刺激分子CD28、CD152、CD80、CD86表达，进一步分析共刺激分子与疾病的关系。方法研究分三组，即健康对照者（HC，n=5）、特发性多发性肌炎组（IPM，n=8）和肌营养不良组（MD，n=8），后两组患者均经肌肉活检酶组织化学确诊。三组患者均通过流式细胞术检测外周血淋巴细胞CD4、CD8、CD4＋CD28＋、CD8＋CD28＋、CD152、CD80、CD86表达，并计算出CD4＋CD28-、CD8＋CD28-表达。结果与HC组比较，IPM组CD4＋CD28＋表达降低（31.9±6.6 vs 39.5±6.9,P〈0.05），其所占CD4＋T细胞的百分率亦明显下降（92.2%±6.2% vs 98.1%±0.7%,P〈0.05），与之相反的是CD4＋CD28-表达明显升高（2.0±1.8 vs 0.6±0.2,P〈0.05）。IPM组CD4、CD8表达及CD8＋CD28＋/CD8百分比与HC组无明显差异，CD8＋CD28-表达则呈上升趋势。IPM组CD152较HC组明显升高（18.5±13.8 vs 7.1±1.2,P〈0.05），各组间CD80表达无明显差异，CD86以IPM组最高（6.8±1.9 vs 4.6±1.7,P〈0.05）。结论IPM患者外周血CD4阳性的T细胞表面的CD28表达下降，但CD152和CD86表达升高。Objective To investigate the expression of CD28, CD152, CD80 and CD86 in peripheral blood lymphoeytes in idiopathic polymyositis, and determine the relationship between these costimu- latory molecules and this disease. Methods Clinical data of healthy controls （HC） （ n = 5）, patients with idiopathic polymyositis （IPM） （ n =8）, muscular dystrophy （MD） （ n =8） were collected. Mus- cular biopsies were carried out on all the patients. The expression of costimulatory molecules CD4＋ CD28＋ , CD8＋ CD28＋ , CD152, CD80 and CD86 in peripheral blood lymphocytes were detected by flow cytometry （FCM）. The expression of CD4＋ CD28- and CD8＋ CD28- were calculated. Results Compared with HC group, the expression of CD4 ＋ CD28 ＋ Tlymphocytes were decreased in IPM group （31.9±6.6 vs 39.5±6. 9, P 〈 0. 05）, and the same pattern was observed in the expression of CD4＋ CD28＋/CD4 （92.2%±6.2% vs 98.%±0. 7%, P 〈 0. 05）. In contrast, the expression of CD4＋ CD28- increased significantly in IPM group compared with HC group （2.0±1. 8 vs 0.6±0.2, P 〈 0.05）. There was no difference in the expression of CD4, CD8 or the percentage of CD8＋ CD28＋/CD8. However, the expression of CD8＋ CD28- had a slight increase. The expression of CD152 in IPM group was higher than in HC group （18.5±13.8 vs 7.1±1.2, P 〈 0. 05）. No differenee in the expression of CD80 was found in the three groups, whereas the expression of CD86 was highest in IPM group （6.8±1.9 vs 4.6± 1.7, P 〈0.05）. Conclusions The expression of CD28 in peripheral blood CD4 positive T lymphoeytes decreased, whereas the expression of CD152 and CD86 increased in idiopathic polymyositis.

关 键 词：特发性多发性肌炎 共刺激分子 CD28 CD152 CD80/CD86 

分 类 号：R773[医药卫生—眼科]