Effect of Emodin in Suppressing Acute Rejection Following Liver Allograft Transplantation in Rats  

作　　者：林胜璋 童洪飞 陈康杰 荆河 杨潇 郑树森 

机构地区：[1]Department of Hepatobiliary Surgery,First Affiliated Hospital of Medical College,Zhejiang University [2]Key Lab of Combined Multi-organ Transplantation,Ministry of Health [3]Department of General Surgery,Second Affiliated Hospital of Wenzhou Medical College [4]Department of General Surgery,Second Affiliated Hospital of Wenzhou Medical College,Wenzhou(325027),China [5]Key Lab of Combined Multi-organ Transplanlation,Ministry of Health,Hangzhou [6]Key Lab of Combined Multi-organ Transplanlation,Ministry of Health

出　　处：《Chinese Journal of Integrative Medicine》2010年第2期151-156,共6页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.30572395,No.30973815).

摘　　要：Objective：To investigate the mechanism of action of emodin for suppressing acute allograft rejection in a rat model of liver transplantation.Methods：Brown Norway（BW） recipient rats of orthotopic liver transplantation（OLT） were divided into three groups,Group A receiving isografting（with BW rats as donor）, Group B receiving allografting（with Lewis rats as donor）,Group C receiving allografting and emodin treatment （50 mg/kg daily）.They were sacrificed on day 7 of post-transplantation,and their hepatic histology,plasma cytokine levels,and T-cell subset expression were detected.Results：Compared with those in Group A,rats in Group B exhibited severe allograft rejection with a rejection activity index（RAI） of 7.67±0.98,extensive hepatocellular apoptosis with an apoptosis index（Al） of 35.83±2.32,and elevated plasma levels of interleukin-2 （IL-2）,interleukin-10（IL-10）,tumor necrosis factor-α（TNF-α）,CD4^＋ and CD4^＋/CD8^＋ ratio.However,recipients in Group C showed a decrease in histological grade of rejection and hepatocellular apoptosis,as well as a decrease in plasma levels of IL-2,TNF-α,CD4^＋ and CD4^＋/CD8^＋ ratio,but elevated levels of IL-10 as compared with the allograft group.Conclusion：Post-OLT acute rejection could be attenuated by emodin,its mechanism of action may be associated with protecting hepatocytes from apoptosis,polarizing the Th 1 paradigm to Th2,and inhibiting the proliferation of CD4^＋ T cell in plasma.Objective：To investigate the mechanism of action of emodin for suppressing acute allograft rejection in a rat model of liver transplantation.Methods：Brown Norway（BW） recipient rats of orthotopic liver transplantation（OLT） were divided into three groups,Group A receiving isografting（with BW rats as donor）, Group B receiving allografting（with Lewis rats as donor）,Group C receiving allografting and emodin treatment （50 mg/kg daily）.They were sacrificed on day 7 of post-transplantation,and their hepatic histology,plasma cytokine levels,and T-cell subset expression were detected.Results：Compared with those in Group A,rats in Group B exhibited severe allograft rejection with a rejection activity index（RAI） of 7.67±0.98,extensive hepatocellular apoptosis with an apoptosis index（Al） of 35.83±2.32,and elevated plasma levels of interleukin-2 （IL-2）,interleukin-10（IL-10）,tumor necrosis factor-α（TNF-α）,CD4^＋ and CD4^＋/CD8^＋ ratio.However,recipients in Group C showed a decrease in histological grade of rejection and hepatocellular apoptosis,as well as a decrease in plasma levels of IL-2,TNF-α,CD4^＋ and CD4^＋/CD8^＋ ratio,but elevated levels of IL-10 as compared with the allograft group.Conclusion：Post-OLT acute rejection could be attenuated by emodin,its mechanism of action may be associated with protecting hepatocytes from apoptosis,polarizing the Th 1 paradigm to Th2,and inhibiting the proliferation of CD4^＋ T cell in plasma.

关 键 词：EMODIN liver transplantation acute allograft rejection apoptosis IMMUNO-SUPPRESSION 

分 类 号：R285.5[医药卫生—中药学]