侧耳属主要种类ITS序列分析  

Analysis of internal transcribed spacer regions of main species of the genus Pleurotus

作　　者：黄晨阳[1] 陈强[1] 高山[1] 高巍[1] 张金霞[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《菌物学报》2010年第3期365-372,共8页Mycosystema

基　　金：公益性行业(农业)科研专项(3-27)

摘　　要：对侧耳属16个种的38个菌株进行了ITS序列测定,统计分析种内和种间序列趋异度。结果表明,16个种的ITS序列种内趋异度很小,为0-0.007,其中Pleurotus djamor不同地理来源的3个菌株的趋异度为0.007。在16个种的种间ITS序列趋异度中,P.rattenburyi和P.djamor之间趋异度最大,为0.282;P.ostreatus和P.cornucopiae之间的趋异度最小,为0.003。利用ITS序列能够对侧耳属的大多种类进行有效鉴定。ITS序列差异2%-3%作为伞菌中种的鉴定临界值在侧耳属中并不合适。而对于P.ostreatus和P.cornucopiae的准确鉴定,仍需开发新的分类鉴定标记。以香菇为外群,用贝叶斯法构建的侧耳属系统发育树表明,系统发育树的部分节点十分可靠,后验概率值为0.96-1.0。In this study, ITS of 38 strains belonging to 16 species of Pleurotus were sequenced, and the sequence divergences were analyzed intraspecifically and interspecifically. The results showed that sequence divergence was low for 16 Pleurotus species, ranging from 0 to 0.007. The sequence divergence was 0.007 for three P. djamor strains from different geographic locations. The 0.282 divergence was the largest, occurring between P. rattenburyi and P. djamor, and the 0.003 divergence was the smallest, occurring between P. ostreatus and P. cornucopiae. Our study indicated that ITS sequencing was a useful tool for identification of most species of Pleurotus. It might not be suitable to identify Pleurotus using the 2%-3% differentiation of ITS sequence which was considered as a critical value for species taxonomical indicator in agaric. New methods are needed to discriminate P. ostreatus and P. cornucopiae. The phylogenetic tree of Pleurotus was yielded from Bayesian analyses compared with an outgroup Lentinula edodes. It showed that partial topology of phylogenetic tree is reliable with posterior probability values 0.96-1.0.

关 键 词：分类鉴定 系统发育 贝叶斯法 序列趋异度 

分 类 号：S646.14[农业科学—蔬菜学]