脆性位点基因FHIT和WWOX在肝细胞癌中的表达及其意义  

The relationship between FHIT and WWOX expression and clinicopathological features in hepatocellular carcinoma

作　　者：林洁[1] 王斌[1] 黄爱民[1] 汪晓军[1] 

机构地区：[1]福建医科大学基础医学院病理学系、福建医科大学肿瘤研究室,福州350004

出　　处：《中华肝脏病杂志》2010年第5期357-360,共4页Chinese Journal of Hepatology

基　　金：福建省教育厅资助项目（7A07088）;福建省自然科学基金（200830286）

摘　　要：目的 探讨脆性位点基因脆性组氨酸三聚体基因（FHIT）和含有WW结构域的氧化还原酶基因（WWOX）在肝细胞癌（HCC）中的表达及其与临床病理特征的关系. 方法采用免疫组织化学染色方法评价142例HCC及其癌旁肝组织和15例正常肝组织中FHIT和WWOX的表达情况,结合临床病理资料进行分析.统计学分析采用Kruskal-Wallis H检验对2或多个独立样本的计数资料或等级资料进行分析,Wilcoxon符号秩检验对2个配对样本的等级资料进行分析,Spearman秩相关进行指标间的相关性分析.结果 68.3%和77.5%的HCC患者中出现FHIT和WWOX表达缺失或下降.肝癌与其相应癌旁组织及正常肝组织表达强度和总水平比较,u值为-9.952～-7.564,P值均＜0.01;且二者的表达具有明显的相关性（P＜0.01）.随HCC分化程度的降低,FHIT和WWOX的表达也减弱（P＜0.05）,并且与血管侵犯、包膜破坏、卫星病灶出现等提示肿瘤侵袭转移的指标有关（P＜0.05）,在术后复发与未复发组中,二者的表达亦有差别（P＜0.05）.结论 FHIT与WWOX在HCC中的缺失或低表达与HCC的恶性表型有关,预示HCC患者的不良预后,二者可能起肿瘤抑制作用.Objective To study the relatonship beteween FHTT and WWOX expression and clinicopathological features in hepatocellular carcinona （HCC）. Method The expression of FHIT and WWOX were determined by immunohistochemistry in 142 patients with HCC. Results Absent or reduced FHIT and WWOX expression was observed in 68.3% and 77.5% of HCCs, respectively. The expression of FHIT was significantly correlated with that of WWOX （P 〈 0.01）, and progressive loss of FHIT and WWOX expression were observed as tumor differentiation decreased and tumor grade increased （P 〈 0.05）. Absent/redused FHIT and WWOX expression was associated with tumor invasion and metastasis （P 〈 0.05）. In addition, the expression of FHIT and WWOX in HCC with recrudesce were lower than that in those without recrudesce （P 〈 0.05）. Conclusions Absent/redused FHIT and WWOX expression is associated with poor prognosis.

关 键 词：癌 肝细胞 染色体脆性位点 脆性组氨酸三聚体基因 含有WW结构域的氧化还原酶基因 

分 类 号：R735.7[医药卫生—肿瘤]