基质金属蛋白酶-9、Ⅳ型胶原、CD34在卵巢上皮性肿瘤中的表达及意义  

Expressions of matrix metalloproteinase-9,IV collagen and CD34 in epithelial ovarian tumor and its significance

作　　者：黄凯清[1] 柯佩奇[3] 梁立治[4] 彭文明[2] 彭娟[2] 刘少颜[2] 

机构地区：[1]广州医学院第三附属医院妇产科,510150 [2]广州医学院第三附属医院病理科,510150 [3]中山大学附属第一医院妇产科 [4]中山大学肿瘤防治中心,广州510060

出　　处：《中国医师进修杂志》2010年第15期1-4,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 探讨基质金属蛋白酶（MMP）-9、Ⅳ型胶原、CD34在卵巢上皮性肿瘤中的表达及意义.方法 卵巢上皮性肿瘤患者82例,其中卵巢恶性上皮性肿瘤48例,卵巢交界性上皮性肿瘤23例,卵巢良性上皮性肿瘤11例.采用免疫组化染色方法,检测MMP-9、Ⅳ型胶原和CD34在肿瘤组织中的表达情况.结果 MMP-9的表达强度随肿瘤恶性程度增加而增强（F=39.306,P〈0.01）.CD34的表达亦随肿瘤恶性程度增加而增强[卵巢良性上皮性肿瘤为（17.18±5.64）%,卵巢交界性上皮性肿瘤为（29.76±7.18）%,高分化卵巢恶性上皮性肿瘤为（57.20±8.55）%,中分化卵巢恶性上皮性肿瘤为（71.20±8.48）%,低分化卵巢恶性上皮性肿瘤为（90.38 ±20.03）%]（F=100.072,P〈0.01）.Ⅳ型胶原在卵巢恶性上皮性肿瘤中的表达强度低于在卵巢交界性上皮性肿瘤和卵巢良性上皮性肿瘤中的表达强度（F=11.554,P〈0.01）. MMP-9表达与CD34表达、Ⅳ型胶原缺失表达均呈正相关（r值分别为0.802和0.796,P〈0.01）.结论 MMP-9、CD34表达随着卵巢上皮性肿瘤恶性程度的增加而增强,Ⅳ型胶原的表达在卵巢恶性上皮性肿瘤中明显减少.MMP-9表达与CD34表达、Ⅳ型胶原缺失表达呈正相关.通过联合检测MMP-9、CD34、Ⅳ型胶原在卵巢上皮性肿瘤中的表达对判断其恶性程度具有重要的临床意义.Objective To explore the expression and significance of matrix metalloproteinase （MMP）-9, IV collagen and CD34 in epithelial ovarian tumor. Methods Eighty-two patients with epithelial ovarian tumor, among them,there were 48 malignant epithelial ovarian carcinomas, 23 borderline epithelial ovarian tumors and 11 benign epithelial ovarian tumors. The expression of MMP-9, IV collagen and CD34 were detected by immunohistochemistry. Results The expression of MMP-9 was strongly linked to the degree of malignant ovarian carcinomas （F= 39.306,P〈 0.01）. The expression of CD34 was also strongly linked to the degree of malignant ovarian carcinomas [benign epithelial ovarian tumors was （17.18±5.64）%,borderline epithelial ovarian tumors was （29.76±7.18）%,well-differentiated malignant epithelial ovarian carcinomas was （57.20±8.55）%,moderately-differentiated malignant epithelial ovarian carcinomas was （71.20±8.48）%, poorly-differentiated malignant epithelial ovarian carcinomas was（90.38±20.03）%]（F= 100.072, P 〈 0.01）. The expression of IV collagen in malignant epithelial ovarian carcinomas was different from that in borderline epithelial ovarian tumors and benign epithelial ovarian tumors （F = 11.554,P〈0.0l）. The expression of MMP-9 was positive correlation with the loss expression of IV collagen and the expression of CD34 （r=0.796,0.802,P〈 0.01）.Conclusions The positive expression of MMP-9,CD34 and the negative expression of IV collagen are obviously relevant to degree of malignant ovarian carcinomas.The combined testing on expression of MMP-9,CD34, IV collagen on ovarian carcinomas is significant to decide degree of malignant ovarian carcinomas and evaluate future development.

关 键 词：卵巢肿瘤 基质金属蛋白酶9 胶原Ⅳ型 抗原 CD34 

分 类 号：R737.31[医药卫生—肿瘤]