mirRNA-17-5p抑制物对骨肉瘤细胞系SOSP_9607增殖和凋亡的影响  

Effects of MicroRNA-17-5p inhibitors on Proliferation and Apoptosis of Human Osteosarcoma Cell Line SOSP-9607

作　　者：赵广义[1] 赵健[1] 高杰[1] 许啸[1] 闫康[1] 吴家昌[1] 杨彤涛[1] 马保安[1] 

机构地区：[1]第四军医大学唐都医院全军骨科中心暨全军骨肿瘤研究所,陕西西安710038

出　　处：《现代生物医学进展》2010年第8期1405-1407,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30672143)

摘　　要：目的:探讨miR-17-5p抑制物对于骨肉瘤细胞系SOSP_9607细胞增殖和凋亡的影响。方法:四甲基偶氮唑蓝(MTT)法测定细胞增殖,进一步计算抑制率,流式细胞仪测定细胞凋亡。将SOSP_9607细胞分为对照组和实验组,对照组分为阴性对照和正常细胞对照组。实验组采用miR-17-5p抑制物(hsa-miR-17-5pinhibitors)抑制SOSP_9607细胞内miR-17-5p的活性。结果:与对照组相比,实验组显著抑制SOSP_9607细胞的增殖,有明显的剂量依赖性(P<0.01)。随着浓度从50nmol/L逐渐增加至200nmol/L,抑制率逐渐增高(P<0.01)。实验组凋亡率(9.6±1.8)%与阴性对照组凋亡率(3.5±0.4)%相比明显增高(P<0.01)。结论:miR-17-5p抑制物通过抑制SOSP_9607细胞中miR-17-5p的活性对SOSP_9607细胞的增殖和凋亡发挥重要作用。Objective：To determine the effects of microRNA-17-5p inhibitors on proliferation and apoptosis of human osteosarcoma cell line SOSP-9607.Methods：MTT assay was used to examine the inhibitive percentage of hsa-miR-17-5p inhibitors on the proliferation of SOSP-9607 cells.Cultured SOSP-9607 cells were divided into two groups：control group and experimental group.The control group contained two groups：the negtive control and the normal control.In experimental group,hsa-miR-17-5p inhibitors were used to inhibit the activity of miR-17-5p.It contained three groups with different doses.The apoptosis and the cell cycle were 1measured by flow cytometry.Cultured SOSP-9607 cells were also divided into two groups：control group and experimental group.Results：Hsa-miR-17-5p inhibitors inhibited SOSP-9607 cell proliferation in a dose-dependent manner（P0.01）,while the control group had no obvious effects on cell proliferation（P0.05）.With the increment of concentration of inhibitors from 50 nmol/L to 200 nmol/L,the inhibition percentage increased significantly （P0.01）.The apoptosis ratio of experimental group（[9.6±1.8）%] was obviously higher than the apoptosis ratio of control group （[3.5±0.4）%]（P0.01）.Conclusions：The hsa-miR-17-5p inhibitors plays an important role in cell proliferation and apoptosis in SOSP-9607 cells by down-regulated the miR-17-5p.

关 键 词：骨肉瘤 成骨肉瘤细胞系SOSP_9607 miR-17-5p 细胞增殖 细胞凋亡 

分 类 号：R738[医药卫生—肿瘤]