基于离子液体共溶剂效应的氯过氧化物酶催化氧化邻苯二胺的研究  

Studies on the Oxidation of σ-Phenylenediamine Catalyzed by Chloroperoxidase in the Presence of an Ionic Liquid as Co-solvent

作　　者：聂艳艳[1] 蒋育澄[1,2] 胡满成[1,2] 李淑妮[1,2] 翟全国[1,2] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062 [2]陕西师范大学大分子科学陕西省重点实验室,西安710062

出　　处：《化学学报》2010年第10期982-988,共7页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20876094)资助项目

摘　　要：通过缓冲液-离子液体混合溶剂中氯过氧化物酶(CPO)催化氧化邻苯二胺(OPD)的产物结构和性能的表征,证实此酶促反应的产物为2,3-二氨基吩嗪(DAP);OPD-H2O2-CPO反应体系有望用于荧光酶联免疫分析;酶动力学分析表明以咪唑类离子液体(ILS)为共溶剂时,CPO对底物的亲和力及对底物识别的专一性都得以改善,从而有效提高了产物收率;酶促反应主要受CPO的稳定性及酶的用量等因素控制.在最佳条件下,产率可达81.16%.The oxidation of σ-phenylenediamine （OPD） catalyzed by chloroperoxidase （CPO） in the pres-ence of an ionic liquids （ILS） as co-solvent was studied in this work. Identifications by UV-Vis,FT-IR and 13C and 1H NMR spectrum show that the product is 2,3-diaminophenazine （DAP）. This ensures the applica-tion of （OPD-H2O2-CPO） system in fluorescence enzyme-link immunoassay. The enzyme kinetic assay in-dicated that both the affinity and specificity of CPO to the substrate was improved in the buffer-ILS mixture,which was the main reason of the enhancement of product yield. This enzyme-catalyzed reaction was con-trolled mainly by the stability and consumption of CPO. The DAP yield was 81.16% under the optimum condition.

关 键 词：氯过氧化物酶 邻苯二胺 2 3-二氨基吩嗪 离子液体 酶动力学 

分 类 号：O625.631[理学—有机化学]