黄嘌呤氧化酶高产菌株发酵培养基的优化  

Optimization of xanthine oxidase high producing strain fermentation medium

作　　者：陈素华[1] 许小平[1] 

机构地区：[1]福州大学化学化工学院,福建福州350108

出　　处：《计算机与应用化学》2010年第5期663-667,共5页Computers and Applied Chemistry

基　　金：福建省自然科学基金资助项目(2008J0100)

摘　　要：为提高生产菌株的产酶水平,本文采用响应面设计方法优化了1株产黄嘌呤氧化酶诱变菌Arthrobacter UW6的培养基组成。首先用Plackett-Burman设计出影响产酶的3个重要的培养基成分:乳糖、黄嘌呤和(NH_4)_2SO_4。然后利用最陡爬坡路径逼近最大响应区域,再通过Box-Behnken设计和响应面分析方法确定出3个主要影响因素的最佳浓度,分别为:乳糖12.83 g/L,黄嘌呤0.7500 g/L,(NH_4)_2SO_4 2.217 g/L。最终酶活力达12.05 U/mL,与初始7.669 U/mL相比,提高了57.13%。响应面法,试验次数少、周期短并能研究多因素间的交互作用,求得因素与响应面值的回归方程并预测最佳值,本文采用此种方法有效地求得产酶菌株的最佳发酵培养基组成。To improve the production of enzyme, response surface methodology （RSM） was constructed to select the mutant Arthrobacter UW6 fermentation medium components of xanthine oxidase. In the first step of optimization, lactose, xanthine and （NH4 ）2SO4 of the media were the main effecting factors while other additives had no significant effect on the yield of xanthine oxidase. Path of steepest ascent was undertaken to approach the optimal region of the significant factors, then through the Box-Behnken design and RSM to determine the optimal concentration of each significant variable. The results showed that the optimum concentrations of lactose, xanthine and （ NH4 ） 2SO4 were 12. 83 g/L, 0. 750 0 g/L, and 2. 217 g/L. As a result, a maximum yield of 12. 05 U/mL was obtained, the enzyme activity increased by 57. 13% under the optimized condition.

关 键 词：黄嘌呤氧化酶 发酵培养基 PLACKETT-BURMAN 响应面分析法 

分 类 号：TQ464.8[化学工程—制药化工]