ROCK1与CD40相互作用的质谱分析及在CD40信号通路中功能的初探  

Mass Chromatographic Analysis of the Interaction between ROCK1 and CD40 and the Preliminary Exploration of the Function of ROCK1 in CD40 Pathway

作　　者：秋昕[1] 方迪峰[1] 高彦飞[1] 甄诚[1] 满江红[1] 梁冰[1] 周涛[1] 李卫华[1,2] 

机构地区：[1]国家生物医学分析中心,北京100850 [2]北京凯正生物工程发展有限责任公司,北京100850

出　　处：《科学技术与工程》2010年第16期3837-3842,共6页Science Technology and Engineering

基　　金：国家自然科学基金(30970593)资助

摘　　要：为鉴定和验证ROCK1和CD40的相互作用,探索ROCK1在CD40通路中可能发挥的作用,应用HD质谱分析ROCK1和CD40的相互作用,用免疫共沉淀实验验证其相互作用,用ROCK1抑制剂研究ROCK1对CD40通路的影响。结果ROCK1与CD40存在相互作用;并参与CD40介导的信号通路。说明ROCK1能与CD40发生相互作用,为CD40信号通路的研究提供了新的线索。To identify and validate the interaction between ROCK1 and CD40 in order to explore the function of ROCK1 in CD40 pathway,the HD MS was applied to screen the protein interacted with CD40 and ROCK1 as an important protein was focused on. The interaction was demonstrated by co-immunoprecipitation assays. The influence of ROCK1 in CD40 pathway was researched by ROCK-specific inhibitor. resulted that ROCK1 is conformed to interact with CD40 and involved in CD40 pathway. is conclusied that ROCK1 interacts with CD40,which may provide an insight on the regulation of CD40 pathway.

关 键 词：ROCK1 CD40 质谱 相互作用 信号通路 

分 类 号：Q78[生物学—分子生物学] R392[医药卫生—免疫学]