HHV-8与非Kaposi肉瘤组织相关性的初步研究  

Preliminary Study on Correlation Between HHV-8 and Non-Kaposi's Sarcomas

作　　者：李振飞[1] 吴秀娟[1] 普雄明[1] 

机构地区：[1]新疆维吾尔自治区人民医院附属皮肤性病医院,新疆乌鲁木齐830001

出　　处：《现代生物医学进展》2010年第9期1673-1675,共3页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2009211A24)

摘　　要：目的:检测人类疱疹病毒8型(HHV-8)在四种非Kaposi肉瘤皮损组织中的感染情况,以初步探讨HHV-8与皮肤鳞状细胞癌、蕈样肉芽肿、Castleman病和化脓性肉芽肿相关性研究。方法:使用酚-氯仿-异戊醇方法对新疆维吾尔自治区人民医院皮肤性病科、病理科从2004年到2009年收集的30例鳞状细胞癌、20例Castleman病(其中5例为多中性Castleman病)、13例蕈样肉芽肿、20例化脓性肉芽肿石蜡包埋组织进行HHV-8DNA抽提;然后使用巢式PCR方法对抽提的DNA进行ORF26基因片段的扩增,扩增产物在1.5%的琼脂糖凝胶上电泳,并在凝胶成像系统下观察电泳结果,从而确定ORF26基因阳性标本;然后计算HHV-8在各种标本中的阳性率。结果:30例皮肤鳞状细胞癌皮损组织有5份为阳性(16.7%)。13例蕈样肉芽肿石蜡组织有1例为阳性(7.7%),20例化脓性肉芽肿和20例Castleman病石蜡组织中均未检出HHV-8。结论:①HHV-8可能是新疆皮肤鳞状细胞癌发病原因之一。②HHV-8与蕈样肉芽肿的发病可能不具有相关性,HHV-8可能是MF发生后偶发的一种附带感染。③Castleman病和化脓性肉芽肿的发病可能均与HHV-8无关。Objective：To study the inflectional situation of ORF26 subtypes of Human herpesvirus-8 in squamous cell carcinoma（SCC）,mycosis fungoides（MF）,Castleman＇s disease（CD）,pyogenic granuloma（PG） in Xinjiang,and to discuss the associations between HHV-8 and different kinds of tissues.Methods：HHV-8 DNA was extracted with traditional phenol-chloroform-isoamyl alcohol from 30 cases SCC,13 cases MF,20 cases CD and 20 cases PG formalin-fixed,paraffin-embedded tissue from the department of dermatologists and pathology of People＇s Hospital of Xinjiang Uygur Autonomous Region from 2004 to 2009.KS is used as controls.The extracted DNA of ORF26 of HHV-8 was amplified by nested PCR.Then PCR product was electrophoresised at the 1.5% agarose gel and ORF26 gene positive samples were observed under the gel imaging system.The analysis of positive specimens of ORF26 gene was carried out.Results：HHV-8 DNA was all detected in 5 cases SCC（16.7%） and in 1 cases MF（7.7%）.There was not HHV-8 in the tissues of PG and CD.Conclusion：HHV-8 infection in SCC of skin has lower rate,but that may be the cause of SCC of skin in XinJiang.NO evidence of HHV-8 infection was found in others three diseases.

关 键 词：人疱疹病毒8型 ORF26基因 化脓性肉芽肿 鳞状细胞癌 蕈样肉芽肿 Castleman's病 

分 类 号：R739.5[医药卫生—肿瘤]