桂皮醛衍生物的合成及其对CVB_3的作用  

Synthesis and anti-coxsackie virus B_3 effects of cinnamaldehyde derivatives

作　　者：刘欣欣[1] 孙晓莉[2] 张松[1] 丁媛媛[3] 王四旺[1] 

机构地区：[1]第四军医大学药学系天然药物学教研室,陕西西安710032 [2]第四军医大学药学系化学教研室,陕西西安710032 [3]北京军区总医院,北京100700

出　　处：《现代生物医学进展》2010年第9期1723-1726,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(20872180)

摘　　要：目的:合成桂皮醛衍生物,观测其对心肌细胞的毒性及抗CVB3病毒的作用。方法:化学合成6种桂皮醛衍生物,其中3种进行了红外、质谱等结构表征;MTT法检测被CVB3病毒感染和用桂皮醛衍生物治疗后的心肌细胞活性。结果:α-溴代对氯肉桂醛、α-溴代对甲基肉桂醛、对氯肉桂醛3种桂皮醛衍生物对心肌细胞的半数毒性浓度(TC50)分别为2151.28μg·mL-1,1475.32μg·mL-1,22460.32μg·mL-1;对CVB3病毒的半数抑制浓度(IC50)分别为178.94μg·mL-1、173.35μg·mL-1、6045.25μg·mL-1;对CVB3病毒的治疗指数(TI)分别为12.02,8.51,3.71。结论:桂皮醛3种衍生物具有直接抑制CVB3病毒作用,但对病毒的细胞合成和吸附无明显作用。Purpose：Cinnamaldehyde derivatives are to be synthesized.Their toxicity to cardiac muscle cells and resistance to virus CVB3 will be observed.Methods：Six Cinnamaldehyde derivatives are chemically synthesized.Three of them are analyzed by infrared spectrometer and mass spectrometer.Activity of cardiac muscle cells which infected by CVB3 and cured by cinnamaldehyde derivatives are measured using MTT.Results：The median toxic concentrations（TC50） of α-bromo-4-chlorocinnamaldehyde,α-bromo-4-methylcinnamaldehyde,4-chlorocinnamaldehyde on cardiac muscle cells are 2151.28μg·mL-1,1475.32μg·mL-1,22460.32μg· mL-1,respectively.The median inhibiting concentrations（IC50） against CVB3 virus are respectively178.94μg·mL-1、173.35μg·mL-1、 6045.25μg·mL-1.And the therapeutic indexes（TI） are respectively12.02,8.51 and 3.71.Conclusion：The three Cinnamaldehyde derivatives have direct repressive effect on virus CVB3,yet no significant effect on its attachment to cell surfaces and production within cells.

关 键 词：CVB3 α-溴代对氯肉桂醛 α-溴代对甲基肉桂醛 对氯肉桂醛 抗病毒作用 

分 类 号：R284.3[医药卫生—中药学] R373[医药卫生—中医学]