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作 者:姚文环[1] 刘艳[2] 冯宪光[3] 韩晓英[4] 孙克任[5]
机构地区:[1]山东省疾病预防控制中心,山东济南250014 [2]中国药品生物制品检定所 [3]山东省交通医院 [4]山东师范大学 [5]山东大学
出 处:《毒理学杂志》2010年第1期24-26,共3页Journal of Toxicology
基 金:山东省科委基金资助项目(981154309)
摘 要:目的研究二十二碳六烯酸(DHA)复合物的抗癌机制。方法以体外细胞培养的方法评价DHA复合物对U251胶质瘤细胞的抑制作用;以RT-PCR方法研究DHA复合物对U251胶质瘤细胞中Bcl-2和Bax mRNA含量的影响。结果DHA复合物U251神经胶质瘤细胞的IC50为0.3746μg/ml(0.3324~0.4168μg/ml);与阴性对照组比较,DHA复合物使U251胶质瘤细胞内Bcl-2 mRNA含量明显降低(P<0.01);Bax mRNA含量明显升高(P<0.01)。结论在本试验条件下,DHA复合物在体外对U251神经胶质瘤细胞有抑制作用,其机制可能为降低Bcl-2基因转录,增加Bax基因转录,从而通过促进细胞凋亡达到抗癌目的。Objective To study the anticancer mechanism of DHA compound.Methods The in vitro effect was assessed by cell-culture.RT-PCR was used to investigate the effects of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma cell.Results The IC50 of DHA compound on U251 glioma cell was 0.3746 μg/ml(0.3324~0.4168 μg/ml);Compared with control,the content of Bcl-2 mRNA was decreasd significantly and content of Bax mRNA was increased significantly in U251 glioma cell after treated with DHA compound.Conclusion The proliferation of the U251 glioma cell was inhibited by DHA compound.The transcrisption of Bcl-2 was decreased and the transcrisption of Bax mRNA was increased in U251 glioma cell after treated with DHA compound and thus the apoptosis of the cancer cell was promoted.
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