H102对APP转基因小鼠突触相关蛋白表达的影响  

Effects of H102 on Synapse-Related Protein Synaptophysin of APP Transgenic Mice

作　　者：王曼[1] 徐淑梅[1] 马志红[1] 

机构地区：[1]天津医科大学生理教研室,300070

出　　处：《天津医药》2010年第5期405-408,450-452,共7页Tianjin Medical Journal

基　　金：天津市科技攻关培育项目(项目编号:05YFGPGX07100)

摘　　要：目的:观察H102对APP转基因小鼠脑内超微结构-突触后致密区(PSD-95)和突触素表达的影响。方法:将16只APP转基因小鼠随机分为模型组和给药组,每组8只。并设同月龄同背景C57BL/6J小鼠为正常组。给药组侧脑室注射H102生理盐水溶液,正常组和模型组侧脑室注射等体积生理盐水,3μL/d,注射30d后行行为学检测即水迷宫测试,然后取出并固定脑组织,利用免疫组化及Western blot方法测定小鼠脑组织突触素、PSD-95及shank1的表达。结果:定位航行实验给药组APP转基因小鼠逃避潜伏期从第2天较模型组差异有统计学意义(P<0.05),较正常组从第3天差异无统计学意义(P>0.05);空间探索实验第三象限停留时间和跨越平台次数较模型组差异有统计学意义(P<0.05),较正常组差异无统计学意义(P>0.05)。模型组皮质和海马出现突触素、PSD-95及shank1表达减少;注射H102后突触素、PSD-95及shank1表达较正常对照组差异无统计学意义(P>0.05)。结论:H102对阿尔茨海默病(AD)治疗有一定的应用价值。Objective：To observe the effect of H102 on synapse-related protein synaptophysin in APP transgenic mice.Methods： The APP transgenic mice were randomly divided into two groups,model group,H102 treatment group,and control group （C57BL/6J mice with the same age and background to model ones）.Mice in model and normal control group were treated with saline solution,while mice of H102 treatment group were treated with compounds （80 μmol/L,H102 in saline solution） injected into the lateral cerebral ventricle once a day for 30 days.Then Morris water maze test was performed.At the end of the experiments mice were killed and immunohistichemical stain and western blot were used to detect the synaptophysin,PSD-95 and shank1.Results： Morris water maze test showed that the escape latency （swimming time to locate the hidden platform） was significantly shorten in H102 treatment group compared with that of model group （P 0.05）;no significant difference between the H102 treatment group and normal group （P 0.05）.There were significant differences in the time and the frequency of crossings platform quadrant 3 between H102 treatment group and model group（P 0.05）;no significant difference between the H102 treatment group and normal group （P 0.05）.The content of synaptophysin,PSD-95 and shank1 were higher in H102 treatment group than those in model group （P 0.05）,and no significant difference pared to the normal group.Conclusion： H102 is a promising treatment for Alzheimer’s disease.

关 键 词：阿尔茨海默病 小鼠 转基因 突触蛋白类 动物实验 小鼠 近交 C57BL PSD-95 shank1 

分 类 号：R749.16[医药卫生—神经病学与精神病学]