应用SYBR greenI荧光PCR研究GSTM1基因多态性与肺癌遗传易感性之间的相关性  

Association between GSTM1 Genetic Polymorphism and Lung Cancer Risk by SYBR Green I Real-time PCR Assay

作　　者：郑德杰[1] 滑峰[1] 梅朝蓉[1] 万海粟[1] 周清华[1] 

机构地区：[1]天津医科大学总医院,天津市肺癌研究所,天津市肿瘤转移与微环境重点实验室,天津300052

出　　处：《中国肺癌杂志》2010年第5期506-510,共5页Chinese Journal of Lung Cancer

基　　金：国家"十一五"科技攻关项目(No.2006BAI02A01);天津市科技支撑计划中瑞合作重大项目(No.09ZCZDSF04100)资助~~

摘　　要：背景与目的谷胱甘肽S转移酶M1(glutathione S-transferase M1,GSTM1)基因是参与体内多种致癌物代谢的重要的II相代谢酶,其基因多态性被认为与人肺癌遗传易感性有关。本研究旨在探讨天津地区汉族人群GSTM1基因多态性与肺癌遗传易感性之间的关系。方法采用SYBR green I实时荧光PCR熔解曲线分析方法检测天津地区265例肺癌患者和307例对照者GSTM1基因多态性,应用病例对照研究分析其与肺癌易感性及不同病理类型之间的关系。结果①GSTM1(-)基因型在肺癌组和对照组的分布频率分别为56.6%和57.0%,两组之间无统计学差别(χ2=0.831,P=0.362)。经性别、年龄、吸烟状况调整后分析,携带GSTM1(-)基因型个体未增加患肺癌危险性(OR=0.840,95%CI:0.578-1.221,P=0.362)。②按病理分层分析GSTM1基因型与肺癌各病理类型之间的关系,其中鳞癌、腺癌、小细胞肺癌与其它病理类型肺癌患者GSTM1(-)基因型分布频率分别为65.8%、48.5%、47.8%和52.2%,与对照组相比,不同病理类型患者肺癌危险性均无明显统计学差异(P>0.05)。结论在天津地区人群中GSTM1基因多态性与肺癌遗传易感性之间无相关性。Background and objective Glutathione S-transferase M1 （GSTM1） is an important phase II metabolic enzyme gene which involves metabolism of various carcinogens in human body. Many studies showed that GSTM1 genetic polymorphism was associated with lung cancer risk. The aim of this study is to investigate the relationship between GSTM1 genetic polymorphism and lung cancer risk among Han nationality population in Tianjin district. Methods GSTM1 genetic polymorphism was detected by melting curve analysis of SYBR green I real-time PCR assay. Two hundred and sixty-five histological confirmed lung cancer patients and 307 health controls were recruited in this case-control study and the relationship between GSTM1 genetic polymorphism and lung cancer risk was investigated. Results （1） The frequency of the GSTM1（-） in lung cancer and control groups was 56.6% and 57.0% respectively, and no significant difference was found between the distribution of the GSTM1（-） genotype in the two groups （χ2=0.831, P=0.362）. （2） When considered the GSTM1（＋） genotype as reference, there was no overall statistically increased lung cancer risk for carriers with the GSTM1（-） genotype adjusted by age, gender and smoking status （OR=0.840, 95%CI： 0.578-1.221, P=0.362）. （3） The frequency of the GSTM1（-） genotype for squamous cell carcinoma, adenocarcinoma, SCLC and other histological types was 65.8%, 48.5%, 47.8% and 52.2% respectively, compared with the control group, no statistically increased lung cancer risk was observed （P0.05）. Conclusion No evidence is found between GSTM1 genetic polymorphism and lung cancer risk among Han nationality population in Tianjin district.

关 键 词：GSTM1 SYBR greenI 基因多态性 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]