共培养系统中巨噬细胞对大鼠视网膜神经节细胞存活和轴突再生的影响  

Effects of macrophages on survival and axonal regeneration of rat retinal ganglion cells in a co-culture model

作　　者：朱彤[1] 崔志利[1] 马丽娜[1] 薛峥[1] 石艳红[1] 惠延年[1] 

机构地区：[1]第四军医大学西京医院眼科,陕西西安710032

出　　处：《中华眼视光学与视觉科学杂志》2010年第2期118-121,共4页Chinese Journal Of Optometry Ophthalmology And Visual Science

摘　　要：目的 观察体外巨噬细胞对视网膜神经节细胞（RGC）存活和生长的影响.方法 采用Transwell小室建立大鼠腹膜腔巨噬细胞和RGC的共培养模型,以不用巨噬细胞共培养的RCC作为对照组,用台盼蓝染色计数及相差显微镜观察RGC存活的时间,并测量培养1 d、3 d和5 d时RGC突起的平均长度,进行组间比较.结果 培养1 d、3 d、5 d和7 d,共培养组平均活细胞数分别为（35.50±2.92）个、（28.20±3.36）个、（18.70±3.95）个和（8.80±1.55）个,对照组为（36.20±2.35）个、（27.10±2.96）个、（15.80±3.04）个和（8.40±2.01）个,两组之间差异均无统计学意义（t=0.369、0.497、0.487、2.854,P均〉0.05）.培养1 d、3 d和5 d,共培养组RGC突起的平均长度分别为（19.79±3.98）μm、（68.30±4.07）μm和（95.51±6.51）μm,明显长于对照组[（15.28±1.28）μm、（58.18±4.22）μm和（82.61±3.75）μm],两组差异均有统计学意义（t=-4.562、-6.554、-7.027,P均〈0.05）.结论Objective To determine the effects of macrophages on the survival and axonal regeneration of retinal ganglion cells （RGC） in vitro. Methods A rat co -culture model was established with RGC and peritoneal cavity macrophages in transwell chambers. RGC cultured without macrophages served as the control. The axonal growth and survival time of RGC in the model were observed under phase contrast microscopy. The survival of RGC was determined by counting the cells with Trypan blue staining. The average lengths of the processes of RGC cultured on days 1, 3 and 5 were measured and calculated. Results The average counts of viable cells with Trypan blue staining in the co-culture system were 35.50±2.92, 28.20±3.36, 18.70±3.95, and 8.80±1.55 on days 1, 3, 5 and 7, respectively. And there were no statistically significant differences when compared to the controls （36.20±2.35, 27.10±2.96, 15.80±3.04, and 8.40±2.01, respectively;t=0.369, 0.497, 0.487, and 2.854; P〉0.05）. The average lengths of the RGC processes in the co-culture system were （19.79±3.98）μm, （68.30±4.07）μn, and （95.51 ±6.5l）μm on days 1, 3 and 5, respectively, which were significantly longer when compared to the controls [（15.28±1.28）μm, （58.18±4.22）μm, and （82.61 ±3.75）μm, respectively; t =-4.562, -6.554, -7.027; P〈0.05）. Conclusion Results shows that macrophages could significantly promote the axonal regeneration of RGC in a co-culture system, but there is no obvious effect on cell survival.

关 键 词：视网膜神经节细胞 巨噬细胞 共培养 视神经再生 

分 类 号：R77[医药卫生—眼科]