牙龈卟啉单胞菌表面相关物质对T淋巴细胞凋亡的影响  

In vitro study of peripheral blood T lymphocyte activation and apoptosis induced by surface-associated material from Porphyromonas gingivalis

作　　者：王惠宁[1] 曹金芳[1] 余杰[1] 

机构地区：[1]温州医学院附属口腔医院口腔内科,温州325027

出　　处：《口腔医学》2010年第5期268-271,共4页Stomatology

基　　金：温州市科技计划项目(Y20080020)

摘　　要：目的研究牙龈卟啉单胞菌表面相关物质(SAM)对人外周血中T淋巴细胞活化及凋亡的作用。方法选取10名全身及牙周组织健康受试者,分离外周血中T淋巴细胞,在有/无SAM情况下培养0～96h,用荧光探针(AnnexinV-FITC、PI、CD69)进行标记,并进行流式细胞仪检测。结果 T淋巴细胞+SAM组AnnexinV+/PI-细胞百分数在48h、72h、96h分别为17.36±2.53,26.48±2.26,46.79±4.53。T淋巴细胞组AnnexinV+/PI-细胞百分数在48h、72h、96h分别为7.15±2.13,6.78±2.15,11.56±2.48,T淋巴细胞+SAM组与T淋巴细胞组之间存在明显差异(P<0.01)。CD69+T淋巴细胞+SAM组和CD69+T淋巴细胞组AnnexinV+/PI-细胞百分数除24h外的4个时间点上都无明显差异(P>0.05)。CD69+淋巴细胞+SAM组AnnexinV+/PI-细胞百分数在各个时间点上都明显高于T淋巴细胞+SAM组(P<0.01)。结论 SAM能够诱导人外周血中T淋巴细胞活化,并且能够通过活化促进其凋亡。Objective To investigate the induction effects of surface-associated material from Porphyromonas gingivalis（SAM）on activation and apoptosis of the human peripheral blood T lymphocytes.Methods Peripheral blood T lymphocytes of healthy volunteers were isolated by Ficoll-Hypaque density gradient centrifugation.Then the cells were incubated with or without surface-associated material from Porphyromonas gingivalis over 0~96 h and the incubation was stopped at 0,24,48,72,96 hours,respectively.The cells were labeled with fluorochrome-conjugated monoclonal antibodies and probes（Annexin V-FITC,PI,CD69）and detected with flow cytometry.Results The percentages of Annexin V＋/PI-cells in the group of T lymphocytes with SAM were 17.36±2.53,26.48±2.26,46.79±4.53 at 48h,72h,96h,respectively;and those in the group of T lymphocytes without SAM were 7.15±2.13,6.78±2.15,11.56±2.48,respectively.The differences in percentages of Annexin V ＋/PI-cells between the two groups were significant（P0.01）.The percentages of Annexin V＋/PI-cells in the group of CD69＋T lymphocytes with SAM and the group without SAM were not significantly different（P0.05）at four time points except at 24 h.The percentages of Annexin V＋/PI-cells in the group of T lymphocytes with SAM were greater than those in the group of CD69＋T lymphocytes with SAM（P0.01）.Conclusion SAM could induce the activation of T lymphocytes and promote the apoptosis through activation.

关 键 词：牙龈卟啉单胞菌表面相关物质 细胞凋亡 淋巴细胞 牙周病 

分 类 号：R780.2[医药卫生—口腔医学]