内源性甲状旁腺激素对于小鼠拔牙创骨愈合影响的研究  

Effects of endogenous parathyroid hormone (PTH) on healing of tooth extraction in mice

作　　者：梁红[1] 陈宁[1] 栾明亮[1] 沈铭[1] 

机构地区：[1]南京医科大学口腔医学研究所,口腔医学院口腔颌面外科,南京210029

出　　处：《口腔医学》2010年第5期276-279,共4页Stomatology

摘　　要：目的探讨内源性甲状旁腺激素(parathyroid hormone,PTH)对小鼠拔牙创骨愈合的影响。方法选用PTH基因敲除小鼠(PTH-/-)和野生型小鼠(PTH+/+)各18只,拔除小鼠双侧上颌第一磨牙,拔牙后第7、14、21天分3批处死小鼠,并通过苏木精-伊红染色、骨总胶原染色、耐酒石酸酸性磷酸酶染色方法进行组织学观察。结果组织学结果显示,与野生型小鼠相比,PTH基因敲除的小鼠拔牙创骨小梁纤细,排列不规则,骨量少,拔牙创愈合延迟,骨面上破骨细胞总长度占骨面长度的百分比降低。结论内源性PTH缺少可以影响小鼠拔牙创愈合。Objective To observe the expression and localization of BMP-2 of condylar cartilage after occlusal vertical rising in adolescent rats.Methods Forty five-week-old male Sprague-Dawley（SD）rats were divided randomly into experimental group（n =20）and control group（n = 20）.Animals in experimental group were fitted with an upper posterior bite rising splints on their upper molars.The animal of experimental group,together with their matched control,were sacrificed 7 d,14 d,21 d and 28d after the appliance insertion（n=5 per group each time）.Sections were cut from right condyles and the expression of BMP-2 in condylar cartilage was examined by immunohistochemicaI analysis.Results In comparison with control group,7 d and 14 d after the appliance insertion,the positive cells of BMP-2 in condylar cartilage were significantly less in the experimental group.28 d after the appliance insertion,the positive cells of BMP-2 in condylar cartilage were significantly more in the experimental group.Conclusions The occlusal vertical rising can lead to a higher expression of BMP-2 in condylar cartilage and the adaptive remodeling of condylar cartilage in adolescent rats.

关 键 词：甲状旁腺激素 拔牙创 创口愈合 

分 类 号：R782.1[医药卫生—口腔医学]