肝细胞癌患者外周血甲胎蛋白mRNA与单核细胞端粒酶活性检测  

Detection of circulation AFPmRNA and telomerase activity in peripheral blood mononuclear cells of patients with hepatocellular carcinoma

作　　者：黄宇琨[1] 范学工[1] 邱氟[2] 王志明[3] 

机构地区：[1]中南大学湘雅医院感染病科,湖南长沙410008 [2]中南大学湘雅三医院移植普通外科,湖南长沙410013 [3]中南大学湘雅医院普通外科,湖南长沙410008

出　　处：《中国普通外科杂志》2010年第5期525-528,共4页China Journal of General Surgery

摘　　要：目的探讨外周血甲胎蛋白(AFP)mRNA和外周血单核细胞(PBMC)端粒酶活性的检测在肝细胞癌(HCC)诊断中的意义。方法采用巢式逆转录PCR(Nest RT-PCR)法检测HCC、肝脏良性肿瘤、慢性肝病患者及正常人外周血的AFP mRNA表达,聚合酶链反应-酶联免疫吸附测定(PCR-ELISA)检测PBMC的端粒酶活性。结果 (1)AFP mRNA在HCC外周血中阳性率为73.3%(22/30),在肝脏良性肿瘤、慢性肝病患者及正常人外周血中无表达(分别为0/10,0/30,0/30),(均P<0.001)。(2)HCC患者PBMC端粒酶活性的阳性率(93.3%,28/30)显著高于肝脏良性肿瘤患者(40%,4/10)和慢性肝病患者(6.7%,2/30)及正常人(0),(分别P<0.01,<0.001,<0.001)。(3)外周血AFP mRNA与HCC PBMC的端粒酶活性表达呈显著正相关(r=0.6742,P<0.05)。结论 AFP mRNA是外周血HCC细胞的特异性标志物;端粒酶活性的检测具有更高的敏感性;两者的联合检测具有重要的临床意义。Objective To investigate the role of detection of circulation alpha-fetoprotein（AFP） mRNA and telomerase activity in peripheral blood mononuclear cells（PBMC） of patients with hepatocellular carcinoma（HCC）.Methods In patients with HCC,benign liver tumors,chronic liver diseases,and healthy volunteers,expression of circulation AFPmRNA and telomerase activity in PBMC were detected using nest reverse transcriptase-PCR（Nest RT-PCR） or PCR-ELISA respectively.Results（1） All the patients with benign liver tumors（n=10）,chronic liver diseases（n=30）,and healthy volunteers（n=30） had negative expression of circulation AFPmRNA,and the positive rate of circulation AFPmRNA in patients with HCC（73.3%,22/30）（all P〈0.001）.（2） In patients with HCC,the positive rate of telomerase activity in PBMC（93.3%,28/30） was significantly higher than those in patients with benign liver tumors（40.0%,4/10）,chronic liver diseases（6.7%,2/30）,and healthy volunteers（0）（P〈0.01,0.001 and 0.001,respectively）.（3） In patients with HCC,circulation AFPmRNA was significantly positively correlated to telomerase activity in PBMC（r=0.6742,P〈0.05）.Conclusions AFPmRNA represents a specific indicator of circulation HCC cells.However,detection of telomerase activity appears to be more sensitive.Combined detection of AFPmRNA and telomerase activity can have important clinical significance.

关 键 词：癌 肝细胞/诊断 甲胎蛋白/血液 端粒酶/血液 巢式逆转录聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]