17β-雌二醇和运动对去卵巢大鼠后肢骨组织PPARγ蛋白表达的影响  

Effects of 17 β-estradiol and exercise on bone PPARγ expression of hinder limb in ovariectomized rats

作　　者：陈永杰[1,2] 沈红[1] 卜淑敏[2] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]首都体育学院科研处,北京100088

出　　处：《中国药理学通报》2010年第5期580-584,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30771046);北京市教委科技发展规划资助项目(NoKM200710029001)

摘　　要：目的观察17β-雌二醇(E2)和运动对去卵巢大鼠后肢骨组织过氧化物酶体增殖物激活受体γ(PPARγ)蛋白表达和骨髓脂肪细胞的影响。方法3mon成年♀性未经产SD大鼠40只,经双侧卵巢切除手术或假手术1wk后,按体重随机分为假手术、去卵巢、去卵巢运动和雌激素4个组。运动组每周进行4次45min、速度18m.min-1、坡度5°的跑台训练。雌激素组每周颈部皮下注射3次,剂量为25μg.kg-1E2。实验结束后,HE染色观察股骨和胫骨形态学变化,Westernblot检测骨组织PPARγ蛋白的表达量。结果去卵巢组股骨远端和胫骨近端的骨髓腔脂肪空泡数目以及股骨PPARγ蛋白表达高于假手术组、去卵巢运动组和雌激素组。结论E2与运动能改善去卵巢大鼠的骨组织学结构,其机制可能部分是与抑制去卵巢大鼠骨组织PPARγ蛋白的表达和抑制骨髓脂肪分化有关。Aim To observe the effects of 17β-estradiol（E2）and exercise on the protein expression of peroxisome proliferators activated receptor γ（PPARγ）in bone tissues and bone marrow adipocytes in ovariectomized rats.Methods Forty female Sprague-Dawley rats,aged three months,were randomly divided into the following four groups：sham-operation（Sham）,ovariectomized（OVX）,ovariectomized plus moderate-intensity treadmill exercise（EX,18 m·minute^-1,45 min·day^-1,5 uphill,4 times·week-1）and ovariectomized treated with E2（25 μg·kg^-1,3 times·week^-1）group.At the end of experiment,the morphological changes of femur and tibia were observed decalcifiedly by HE staining and the protein expression of bone PPARγ was detected by Western blot.Results The number of fat vacuoles and PPARγ protein expression in OVX group was significantly higher than that of other three groups.Conclusion E2 and exercise can benefit the bone morphological structure and these effects might be partly related to the inhibition of both PPARγ protein expression in bone tissues and adipogenic differentiation of bone marrow in OVX rats.

关 键 词：17Β-雌二醇 运动 PPARΓ 去卵巢大鼠 骨质疏松 骨髓干细胞 

分 类 号：R-332[医药卫生] R323.72