玉屏风多糖对佐剂性关节炎大鼠T细胞亚群的影响  

The influence of Yupingfeng polysaccharide on T lymphocyte subgroup of adjuvant arthrits rat

作　　者：陈莹瑛[1] 李俊[1] 施磊[1] 王佳佳[1] 黄艳[1] 

机构地区：[1]安徽医科大学药学院,安徽天然药物活性研究省级实验室,安徽合肥230032

出　　处：《中国药理学通报》2010年第5期588-592,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30973917);国家中医药管理局中医药科研三级实验室“中药药理实验室”(NoTCM-2009-197)

摘　　要：目的观察玉屏风多糖(Yupingfeng Polysaccharide,YPF-P)对佐剂性关节炎大鼠T细胞亚群的影响。方法采用弗氏完全佐剂(FCA)诱导大鼠AA模型。足容积法测量继发侧足肿胀度;MTT法检测刀豆蛋白(ConA)诱导的外周血淋巴细胞增殖反应;流式细胞仪检测大鼠外周血中CD4+和CD8+T细胞的变化;ELISA法检测大鼠外周血中干扰素γ(interferon-γ,IFN-γ)含量;放免法检测大鼠外周血中IL-4含量;RT-PCR法检测淋巴细胞IFN-γmRNA的表达。结果80、160mg.kg-1YPF-P给药能明显减轻关节肿胀度,不同程度地改善AA大鼠低下的外周血淋巴细胞增殖反应,明显降低CD4+/CD8+的相对比值。此外,80、160mg.kg-1YPF-P给药也能明显降低外周血IFN-γ含量和轻度提高IL-4含量,并能抑制IFN-γmRNA的表达。结论YPF-P能改善模型大鼠外周血T细胞亚群,纠正佐剂性关节炎模型大鼠Th1/Th2平衡的Th1偏移,这可能是减轻足肿胀的原因之一。Aim To explore the effects of Yupingfeng polysaccharide（YPF-P）on T cells of adjuvant arthritis（AA）in rats.Methods Freund＇s complete adjuvant was used to induce AA in rat.Secondary paw swelling of AA rats was measured with volumeter.Peripneral blood lymphocyte proliferation response induced by concanavalin A（ConA）was examined with MTT assay.The CD4^＋ and CD8^＋ T cell in peripheral blood were detected by flow cytometer.ELISA method was applied to determine the content of factor IFN-γ and radio-immunity assay of factor IL-4.RT-PCR procedures were utilized to determine the expression of IFN-γ mRNA.Results 80,160 mg·kg^-1 YPF-P could singnificantly inhibit the secondary paw swelling of AA rats.The suppressesion of lymphocyte proliferation of peripheral blood T cell in AA rats was reversed.After treatment by YPF-P,the number of CD4^＋ cell and the ratio of CD4^＋/CD8^＋ markedly decreased.The level of IFN-γ in YPF-P treating group was obviously decreased in comparison with that of model control group,while the level of IL-4 was increased.The mRNA expression of IFN-γ was inhibited as well.Conclusion Yupingfeng polysaccharide can regulate the turbulence of periphery abnormal T cell subgroups of adjuvant arthritis rats and can recover Th1/Th2 balance to move towards Th1,which may be one of its mechanisms of inhibiting the secondary paw swelling.

关 键 词：玉屏风多糖 佐剂性关节炎 T细胞亚群 IFN-Γ IL-4 RT-PCR 

分 类 号：R-332[医药卫生] R284.1