肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变  

Alteration of [Ca^(2+)]_i in pulmonary artery smooth muscle of pulmonary artery hypertension rats

作　　者：承伟[1] 李智[2] 

机构地区：[1]辽宁医学院药学院,辽宁锦州121000 [2]中国医科大学药学院天然药物研究室,辽宁沈阳110001

出　　处：《中国药理学通报》2010年第5期593-596,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No30470765)

摘　　要：目的分析肺动脉高压时肺动脉平滑肌细胞Ryanod-ine受体[Ca2+]i释放功能的改变。方法腹腔注射野百合碱建立大鼠肺动脉高压模型,原代培养肺动脉平滑肌细胞,Fura-2/AM负载培养细胞,荧光测钙技术测量Ryanodine受体激动剂对[Ca2+]i变化的影响。结果10nmol.L-1Ry-anodine使对照组[Ca2+]i平均增加(93.31±12.41)nmol.L-1,使PAH组[Ca2+]i平均增加(141.71±13.59)nmol.L-1。两组样本[Ca2+]i增加的数值差异有显著性(P<0.01);10mmol.L-1Caffine使对照组[Ca2+]i平均增加(149.02±13.02)nmol.L-1,使PAH大鼠PASMC的[Ca2+]i平均增加(191.2±21.26)nmol.L-1,两组样本[Ca2+]i数值的变化差异有显著性(P<0.01)。结论肺动脉高压大鼠原代培养的肺动脉平滑肌细胞对Ryanodine受体激动剂的敏感性增强,提示肺动脉高压时Ryanodine受体释放[Ca2+]i的功能发生了异常改变。Aim To investigate the changes of [Ca^2＋]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol·L^-1 Ryanodine,the concentration of [Ca^2＋]i in control group increased by（93.31±12.41）nmol·L^-1;and the concentration in PAH group increased by（141.71±13.59）nmol·L^-1（P0.01）;10 mmol·L^-1Caffine could increase [Ca^2＋]i in control group by（149.02±13.02）nmol·L^-1,and increase [Ca^2＋]i in PAH group by（191.2±21.26）nmol·L^-1（P〈0.01）.Conclusion The results demonstrate that the sensitivity of PASMCs of PAH rats to RyR agonist increase markedly,and suggest that the RyR Ca^2＋ release probably is abnormal in pulmonary artery smooth muscle cells of PAH rats.

关 键 词：肺动脉高压 RYANODINE受体 肺动脉平滑肌细胞 细胞内钙 激动剂 荧光测钙技术 

分 类 号：R-332[医药卫生] R322.121