氟哌啶醇致新西兰兔QT间期延长及其对L型钙通道mRNA表达水平的影响  

Haloperidol induced long QT interval and effects on L-type calcium channel mRNA expression of New Zealand rabbit

作　　者：阿迪力.阿不都热合曼[1] 闫冬[1] 邹洲[1] 帕尔哈提.克热木 

机构地区：[1]新疆医科大学药学院药理学教研室,新疆乌鲁木齐830011

出　　处：《中国药理学通报》2010年第5期615-618,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(2004年;No30460147);新疆自治区高校科研计划项目(NoXJEDU2005Ⅰ14)

摘　　要：目的观察不同剂量氟哌啶醇对新西兰兔心电图QT间期的影响及其对L型钙通道mRNA表达水平的影响。方法采用体表心电图技术,通过BL-410生物机能实验系统动态记录Ⅱ导联心电图的变化,观察不同剂量氟哌啶醇对新西兰兔心电图的影响。提取心肌组织总RNA,采用RT-PCR方法,观察氟哌啶醇对L型钙通道mRNA表达水平的影响。结果氟哌啶醇能引起的QT间期延长,剂量越大延长越明显。氟哌啶醇对QT间期的影响主要发生在给药后0～120min,360min后基本恢复正常。RT-PCR结果显示氟哌啶醇使L型钙通道mRNA表达水平明显增高。结论氟哌啶醇可使QT间期延长,其机制可能与增强L型钙通道mRNA表达有关,从而增加发生室性心律失常的潜在危险性。Aim To observe the influence of haloperidol on QT interval and effects on L-type calcium channel mRNA expression.Methods By surface ECG technique and RT-PCR technique observe the influence of Various doses haloperidol to New Zealand rabbits ECG.And L-type calcium channel mRNA.Results Haloperidol elongated the QT interval.Haloperidol influence QT interval mainly happened after from 0 minutes to 120 minutes.360 minutes later,QT interval turned to normal standard.RT-PCR result display haloperidol obviously increase L-type calcium channel mRNA expression.Conclusion Haloperidol can prolong the QT interval.and it＇s mechanism concerned with increase L-type calcium channel mRNA expression.So,it can increase the risk of ventricular arrhythmias occur.

关 键 词：氟哌啶醇 新西兰兔 心律失常 QT间期 RT-PCR mRNA 

分 类 号：R-332[医药卫生] R322.11