Salusin-β对血管平滑肌细胞增殖的作用及其机制研究  

Effect of salusin-β on the proliferation of vascular smooth muscle cells and the underlying mechanisms

作　　者：刘兰兰[1] 武玉清[1] 宋征[1] 周成华[1] 邢淑华[1] 

机构地区：[1]徐州医学院药学院药理学教研室,江苏徐州221004

出　　处：《中国药理学通报》2010年第5期666-669,共4页Chinese Pharmacological Bulletin

基　　金：徐州医学院院长专项基金资助项目(No08KJZ06);徐州医学院研究生科技创新计划资助项目(NoXYCX200920)

摘　　要：目的研究新的内源性活性肽salusin-β对血管平滑肌细胞(VSMCs)增殖的影响及其作用机制。方法原代培养大鼠主动脉VSMCs,MTT法测定不同浓度salusin-β(0.1、1、10nmol.L-1)对VSMCs增殖的影响;Westernblot法测定p-ERK1/2蛋白表达;RT-PCR测定c-myc、c-fos基因表达。结果与对照组相比,salusin-β明显刺激大鼠VSMCs增殖,增加p-ERK1/2蛋白表达,促进c-myc、c-fos基因表达。结论Salusin-β促进大鼠VSMCs增殖可能与激活ERK1/2途径有关。Aim To observe the effect of salusin-β on the proliferation of vascular smooth muscle cells（VSMCs）and its underlying mechanisms.Methods Primary VSMCs were cultured by the tissue explants method.The effect of salusin-β（0.1,1,10 nmol·L^-1）on VSMCs proliferation was determined by MTT assay.The expression of phospho-ERK1/2 was detected by Western blot analysis.The mRNA expressions of c-myc and c-fos were measured by RT-PCR.Results Compared with control group,salusin-β significantly stimulated the proliferation of VSMCs,increased the protein expression of phospho-ERK1/2,and promoted the mRNA expressions of c-myc and c-fos.Conclusion ERK1/2 pathway may be involved in the effect of salusin-β on the proliferation of VSMCs.

关 键 词：salusin-β 肽 血管平滑肌细胞 增殖 ERK1/2 C-MYC C-FOS 

分 类 号：R-332[医药卫生] R322.74