胆碱与叶酸不同浓度组合诱导正常人与乳腺癌患者成淋巴细胞凋亡效应的比较  

Comparaison of lymphoblast apoptosis induced by different combinations of choline and folate between healthy and breast cancer individuals

作　　者：高微微[1] 徐伟江[1] 周滔[1] 汪旭[1] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092

出　　处：《癌变．畸变．突变》2010年第3期210-213,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30760098;30960166);云南省社会发展基础研究计划(2008C008)

摘　　要：目的:比较携带BRCA1基因突变的乳腺癌患者与正常人成淋巴细胞株对胆碱(cholinechloride,CC)与叶酸(folicacid,FA)缺乏诱发细胞凋亡的差异。方法:实验设置了6个CC(0～21.5μmol/L)与4个FA(30～240nmol/L)的24种不同浓度的组合,培养乳腺癌患者BRCA1基因突变的人成淋巴细胞株GM13705(病例组)和人成淋巴细胞株GM12593(正常对照组),9d后,用胞质阻断微核细胞组分析(CBMNCyt),比较胆碱与叶酸不同浓度组合中两种细胞株的凋亡率。结果:当CCC≥6μmol/L、CFA≥120nmol/L时,两细胞株的凋亡率均显著降低(P<0.01或P<0.05)。在所有浓度组合中,病例组细胞株的凋亡率均比对照细胞株低,但消减背景值后,两细胞株的凋亡率差异均无统计学意义(P>0.05)。结论:乳腺癌患者人成淋巴细胞株对胆碱和叶酸的凋亡效应无特殊敏感性。OBJECTIVE：To compare the difference on the frequency of apoptosis induced by choline（CC）and folic acid（FA）deficiency in lymphoblast cell lines from breast cancer patient carrying BRCA1 genetic mutation and healthy control.METHODS：Lymphoblast cell lines from breast cancer patient carrying BRCA1 genetic mutation and healthy control were cultured under different media with 24 concentration combination of CC（0～21.5 μmol/L）and FA（30～240 nmol/L）for 9 days.Apoptosis（APO）frequencies of two cell lines was analysed by cytokinesis-block micronucleus cytome assay（CBMN Cyt）.RESULTS：The APO frequencies of two cell lines were significantly decreased at and over 6 μmol/L CC or 120 nmol/L FA（P〈0.01 or P〈0.05）.The APO frequencies in cell line of case group were lower than the control cell line in all combinations.After the genetic damage background was reduced,there was no statistical significance（P〉0.05）in APO frequencies difference of two cell lines.CONCLUSION：Apoptosis of lymphoblast cell lines from breast cancer patients was not more sensitive to CC and FA than control group.

关 键 词：胆碱 叶酸 胞质阻断微核细胞组分析 成淋巴细胞 

分 类 号：R737.9[医药卫生—肿瘤]