STAT3、VEGF基因在新疆哈萨克族食管癌中的表达及其相关性研究  

Expression of STAT3,VEGF in esophageal carcinoma and its correlation in Xinjiang Kazaks

作　　者：王洪江[1] 郭文佳[2] 李卉[3] 庞作良[1] 陈艳[2] 姜孝芳[3] 张景萍[2] 张丽萍[2] 范志勤[1] 赵学信[2] 李惠武[3] 

机构地区：[1]新疆医科大学附属肿瘤医院胸外科 [2]新疆医科大学基础医学院生物化学教研室 [3]新疆医科大学基础医学院科研中心新疆地方病分子生物学实验室,乌鲁木齐830011

出　　处：《癌变．畸变．突变》2010年第3期218-220,237,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区高技术研究与发展计划项目(200810103)

摘　　要：目的:研究信号转导和转录激活因子3(signal transducer and activator of transcription3,STAT3)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)基因在哈萨克族食管癌中的表达及相关性,从而为探讨哈萨克族食管肿瘤癌变的机制奠定基础。方法:采用RT-PCR方法检测STAT3、VEGF基因在30例哈萨克族食管癌组织及相应癌旁组织(手术切缘无癌组织)中的表达。结果:STAT3、VEGF基因在30例食管癌组织中表达的阳性率分别为73.33%(22/30)和76.67%(23/30),30例癌旁组织中表达的阳性率为43.33%(13/30),两种基因在癌组织中表达的阳性率均高于癌旁组织(P<0.05)。STAT3与VEGF基因的表达具有相关性(P<0.05)。结论:STAT3、VEGF基因的表达可能与哈萨克族食管癌的发生有关,两者之间可能具有协同作用,共同促进食管癌的发生。OBJECTIVE：To study the expressions of STAT3 and VEGF gene and to explore their roles in the process of esophageal cancer in the Kazaks.METHODS：Using RT-PCR to detect STAT3,VEGF in 30 cases of esophageal cancer tissues and their corresponding adjacent tissues.RESULTS：The positive rate of STAT3,VEGF mRNA expressions were 73.33%（22/30）and 76.67%（23/30）,respectively,in the esophageal cancers,and 43.33%（13/30）in adjacent tissues.There was significant difference in the expressions of STAT3,VEGF mRNA level between tumor tissue and adjacent tissues.CONCLUSION：STAT3,VEGF gene could have important roles in carcinogenesis of esophageal cancer in the Kazaks.The combined action of STAT3 and VEGF genes could promote carcinogenesis and development of esophageal cancer.

关 键 词：哈萨克族食管癌 转导和转录激活因子3 血管内皮细胞生长因子 逆转录聚合酶链式反应 

分 类 号：R735.1[医药卫生—肿瘤]