siRNA干扰ERK1/2表达对食管癌细胞ezrin基因的转录调控作用  

Effect of ERK1/2 siRNA on Transcriptional Regulation of ezrin in Esophageal Carcinoma Cells

作　　者：高书颖[1,2] 李恩民[1] 陆晓峰[3] 杜则澎[1] 许丽艳[3] 

机构地区：[1]汕头大学医学院生物化学与分子生物学教研室,515041 [2]深圳北京大学香港科技大学医学中心 [3]汕头大学医学院肿瘤病理研究室

出　　处：《肿瘤防治研究》2010年第5期491-494,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30672376;30570849;30370641);广东省自然科学基金资助项目(05104541;7301043)

摘　　要：目的检测siRNA干扰细胞外信号调节激酶ERK1/2基因表达对ezrin基因的转录调控作用,探讨食管癌细胞ezrin基因的表达调控机制。方法采用定量RT-PCR技术,检测转染ERK1/2siRNA对食管癌EC109细胞ERK1/2和ezrin基因mRNA表达水平的影响;采用双荧光素酶报告基因分析系统,检测siRNA干扰ERK1/2表达对EC109细胞ezrin基因启动子活性的影响。结果在食管癌EC109细胞中,转染ERK1/2siRNA,降低ERK1/2和ezrin基因的mRNA表达水平以及ezrin基因启动子活性。结论 ERK1/2对食管癌细胞ezrin基因的转录具有调控作用。Objective To identify the effect of ERK1/2 siRNA on the transcriptional regulation of ezrin gene and elucidate the transcriptional regulatory mechanism of ezrin in human esophageal carcinoma cells.Methods Esophageal carcinoma EC109 cells were transfected with ERK1/2 siRNA or control siRNA,and the effect of siRNA on ERK1/2 and ezrin genes mRNA expression was determined by real time RT-PCR.Furthermore,the role of ERK1/2 siRNA interference in ezrin promoter activity was analyzed by dual-luciferase reporter assay system.Results Transfection of ERK1/2 siRNA to EC109 cells decreased the mRNA expression of ERK1/2 and ezrin genes and the ezrin promoter activity.Conclusion ERK1/2 regulate ezrin gene transcription in human esophageal carcinoma cells.

关 键 词：EZRIN基因 食管癌细胞 定量RT-PCR 细胞外信号调节激酶1/2 荧光素酶活性检测 

分 类 号：R735.1[医药卫生—肿瘤]