酵母双杂交筛选原癌基因proto-Dbl的上游调控分子  

Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System

作　　者：唐秋琳[1] 赵晓斐[1] 陈向征[1] 刘明[1] 陈济[1] 毕锋[1] 

机构地区：[1]四川大学华西医院信号转导与分子靶向治疗研究室/腹部肿瘤科,成都610041

出　　处：《肿瘤防治研究》2010年第5期499-502,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30971519);教育部博士点基金资助项目(20060610068)

摘　　要：目的探讨VL domain对Dbl癌基因活性的调控机制。方法本文通过酵母双杂交的方法 ,以proto-Dbl的VL domain为诱饵蛋白对人胎脑文库进行筛选。结果研究共获得7个能与VL domain相互作用的蛋白。结论 Proto-Dbl VL domain相互作用蛋白的发现将为揭示proto-Dbl的功能奠定基础。Objective The VL domain truncate mutation could directly cause the activation of proto-Dbl and the malignant transformation.Methods In order to clarify the activation mechanism of proto-Dbl,yeast two-hybrid system was used to screen the proteins interacted with the VL domain in the human fetal brain cDNA library taking VL domain as the bait.Results 7 positive clones which interact with proto-Dbl-VL domain were successfully harvested.Conclusion This discovery lays the foundation for uncovering of the regulatory mechanism of proto-Dbl.

关 键 词：原癌基因 Dbl癌基因 酵母双杂交 

分 类 号：R733.4[医药卫生—肿瘤]