氟达拉宾联合瘤苗抗小鼠RMA淋巴瘤的实验  

Experiment of Anti-RMA Lymphoma of Mice by Fludarabine Peritoneal Injection Associated with Inactivated Lymphoma Vaccine

作　　者：李翔[1] 高全立[2] 买玲[3] 

机构地区：[1]郑州大学基础医学院病理学与病理生理学系,450052 [2]河南省肿瘤医院血液科 [3]河南省肿瘤医院科研外事科

出　　处：《肿瘤防治研究》2010年第5期519-521,共3页Cancer Research on Prevention and Treatment

基　　金：河南省省属科研机构2006年度研究开发专项资金资助项目(0641130201)

摘　　要：目的研究注射用化疗药物氟达拉宾(Fludarabine)联合瘤苗治疗小鼠RMA淋巴瘤的作用与机制。方法以C57BL/6小鼠为研究对象,通过Western blot分析实验组与对照组小鼠脾脏组织中Foxp3基因的表达,并观察各组小鼠的肿瘤生长与生存期。结果氟达拉宾组与对照组相比,能够下调Foxp3基因的表达。氟达拉宾联合瘤苗能有效抑制肿瘤的生长速度,并提高荷瘤小鼠的生存期,与对照组相比差异有统计学意义(P<0.05)。结论氟达拉宾联合瘤苗能够增强小鼠机体抵抗肿瘤的能力,并能够延长小鼠生存期。Objective To observe the anti-RMA lymphoma effect and mechanism by Fludarabine injection associated with inactivated lymphoma vaccine.Methods Use C57BL/6 mice as a target,Foxp3 genes in spleens were identified by Western blot,at the same time,the tumor growth and survival of the Fludarabine group and the control group were observed.Results Fludarabine could downregulate the expression of Foxp3 gene,and fludarabine associated with vaccine could not only inhibit the tumor growth but also improve survival of animals（P〈0.05）.Conclusion The experiment revealed that Fludarabine associated with vaccine can strengthen the ability to antagonize tumor,and prolog life span of tumor-bearing mice.

关 键 词：氟达拉宾 瘤苗 FOXP3 免疫增强作用 

分 类 号：R733.4[医药卫生—肿瘤]