钾通道KCNQ1在大鼠胰腺发育过程中的表达  

Expression of KCNQ1 channel during rat pancreas development

作　　者：刘莉洁[1] 石丽娟[1] 王莉[1] 郭静[2] 德伟[2] 

机构地区：[1]东南大学医学院生理学教研室,南京210009 [2]南京医科大学生物化学与分子生物学系,南京210029

出　　处：《第二军医大学学报》2010年第5期472-475,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30770537)~~

摘　　要：目的分析钾通道KCNQ1在不同发育阶段大鼠胰腺以及成年大鼠胰岛中的表达情况,为探悉其在胰腺B细胞发育及功能中可能的作用提供实验依据。方法采用高密度寡核苷酸芯片对胚胎第12.5天(E12.5)、E15.5、E18.5、新生和成年大鼠胰腺进行基因转录水平分析,并用RT-PCR对KCNQ1通道亚基在上述5个时期大鼠胰腺以及成年大鼠胰岛中的表达进行验证和分析。结果与胰腺内分泌细胞分化、成熟有关的转录因子分别于E15.5、E18.5达到表达高峰;E18.5胰腺中B细胞功能成熟标志基因的表达显著增加,而KCNQ1钾离子通道的编码基因KCNQ1及KCNE1此时才出现表达。成年胰腺及胰岛中均具有多种KCNQ1通道亚基的表达。结论 KCNQ1通道的表达出现于胰腺发育后期内分泌细胞功能逐渐成熟阶段,在成年胰岛中亦具有较高表达水平,提示KCNQ1通道可能表达于成熟B细胞并参与其内分泌功能。Objective To investigate the dynamic expression profile of KCNQ1 channel in rat developing pancreas and adult rat islets,so as to provide experimental basis for further analyzing the possible role of KCNQ1 channel in β-cell development and function.Methods The gene expression patterns of embryonic day 12.5 （E12.5）,E15.5,E18.5,new-born and adult rat pancreas were compared using the GeneChipRAE 230A.The expressions of KCNQ1 channel subunits in rat pancreas at the above five stages and adult rat islets were also detected and analzyed by RT-PCR.Results The results of genechip showed that the expression of transcription factors related to endocrine cell differention and maturation peaked at E15.5 and E18.5,respectively.The expression of mature β-cell specific genes increased significantly at E18.5,but KCNQ1 and KCNE1 only started to express at E18.5.The results of RT-PCR showed expression of multiple KCNQ1 channel subunits in adult rat pancreas and islet.Conclusion The expression of KCNQ1 channel appears in the later stage of pancreatic development when the endocrine cells undergo further maturation,and has a higher level in adult islets.The expression profile of KCNQ1 channel subunits indicates that KCNQ1 channel might be expressed in mature β-cell and involved in its endocrine function.

关 键 词：KCNQ1钾通道 胰岛 胰腺 发育 

分 类 号：R339.35[医药卫生—人体生理学]