视网膜神经节细胞轴突再生相关神经胶质酸性蛋白和生长相关蛋白-43基因研究  

Changes in glial fibrillary acidic protein and growth-associated protein-43 expressions in retinal ganglial cells during axonal regeneration

作　　者：曾勇[1] 万静[1] 万琨[1] 李圆圆[1] 李力燕[2] 王廷华[2] 冯忠堂[2] 金顺祥[3] 李燕[1] 

机构地区：[1]昆明医学院第一附属医院 [2]昆明医学院神经科学研究所 [3]昆明市儿童医院眼科,云南昆明650031

出　　处：《南方医科大学学报》2010年第4期789-792,共4页Journal of Southern Medical University

基　　金：云南省科技厅国际合作项目(2005GH24)

摘　　要：目的探索视神经移植后,再生的视网膜神经节细胞中基因表达的变化。方法将39只Listed Hooded大鼠分为正常对照组、神经截断组和截断+移植组。采用外周神经缝接模型,用免疫组化技术检测GFAP、GAP-43在各组不同时期表达的变化。通过采用RT-PCR技术检测大鼠视网膜中13个基因的mRNA浓度的变化,使用ANOVA进行统计比较。结果视网膜切片显示了神经截断组GFAP有明显的表达上调;截断+移植组的GFAP表达下调,GAP-43在不同时点在GCL出现不同程度的表达上调。Real-time PCR结果,5d后,视网膜中的GFAP、GAP-43表达在截断组和截断+移植组与对照组相比有显著的增加。在其后的时点,GAP-43的表达在两个实验组都比对照组有显著降低。结论再生的视网膜可能调节GFAP合成。再生的RGCs表达GAP-43。GAP-43上调的结果为神经再生提供了依据。Objective To explore the changes in the expressions of glial fibrillary acidic protein （GFAP） and growth- associated protein-43 （GAP-43） in retinal ganglial cells after neural transplantation.Methods Thirty-nine rats were randomized into normal control group,nerve amputation group and nerve amputation with peripheral nerve transplantation group.Immunohistochemistry was used to detect the changes in the expressions of GFAP and GAP-43 at different time points after the operations,and real-time PCR was employed to detect the mRNA expressions of 13 genes in the retinal ganglial cells of the rats.Results Immunohistochemistry showed obviously increased GFAP expressions in the retina following the nerve amputation.GFAP expression was down-regulated while GAP-43 expression upregulated in the retinal ganglial cells after peripheral nerve transplantation.Real-time PCR results showed that 5 days after the operations,retinal GFAP and GAP-43 expressions increased significantly in the nerve amputation group and peripheral nerve transplantation groups as compared with those in the control group,but GAP-43 expression decreased significantly in the former two groups afterwards.Conclusions The regenerated retina may adjust the production of GFAP.The retinal ganglial cells express GAP-43 during retinal regeneration.Up-regulation of the expression of GAP-43 provides the evidence for nerve regeneration following the nerve transplantation.

关 键 词：神经再生 生长相关蛋白-43 神经胶质酸性蛋白 

分 类 号：R774.1[医药卫生—眼科]