内皮素A受体拮抗剂在人喉癌种植瘤的实验研究  

Effects of BQ-123 on Carcinoma Angiogenesis of Implanted Laryngeal Carcinoma

作　　者：林雁[1] 张京晶[2] 冯骏[1] 刘世喜[1] 

机构地区：[1]四川大学华西医院耳鼻咽喉头颈外科,四川成都610041 [2]昆明医学院公共卫生学院,云南昆明650031

出　　处：《华西医学》2009年第10期2674-2676,共3页West China Medical Journal

基　　金：四川省科技厅应用基础研究项目2008JY0026

摘　　要：目的:探讨选择性内皮素A受体拮抗剂BQ-123对人喉癌Hep-2细胞裸鼠种植瘤的生长及血管形成的影响。方法:将实验动物裸鼠随机分为3组:BQ-123[n=8,2mg/(kg.day)]、氟尿嘧啶组[n=8,2mg/(kg.day)]、生理盐水组(n=8),比较各组裸鼠成瘤体积、微血管密度(MVD)。结果:BQ-123组肿瘤体积为(1.62±0.53)cm3,明显小于生理盐水组及氟尿嘧啶组,差异具有统计学意义;BQ-123组的肿瘤组织中MVD高倍镜下为23.2,明显低于生理盐水组(58.6)及氟尿嘧啶组(39.5),差异具有统计学意义。结论:BQ-123对人喉癌Hep-2细胞在裸鼠体内有明显抑瘤作用,肿瘤的体积、肿瘤组织MVD显著低于对照组,表明BQ-123可通过抑制肿瘤血管生成而显著抑制肿瘤生长。Objective：To study the effects of endothelin A receptor blockade BQ-123 on the implanted human laryngeal carcinoma angiogenesis of nude mouse.Methods：From March 2008 to July 2009,24 Balb/c nude mice were randomly divided into three groups：BQ-123 group [n=8,BQ-123 at 2 mg/（kg·day）],5-Fu group [n=8,fluorouracil at 2 mg/（kg·day）] and the control group （n=8,normal saline）.The carcinoma volume and microvascular density of each group were compared.Results：The tumor size of BQ-123 group,which was （1.62±0.53）cm^3 in average,was significant smaller than the tumor sizes of the other two group s.The average microvascular density score of the tumors in BQ-123 group was 23.2 per hyper power len （HP）,which was also significantly less than the average scores of control groups （58.6 and 39.5 respectively）.Conclusion：Nude mouse experiments show that the carcinoma volume and microvascular density of BQ-123 group are significantly lower than those of the control groups.BQ-123 inhibits the growth of carcinoma by its inhibition of carcinoma angiogenesis.

关 键 词：喉癌 血管形成 微血管密度 内皮素A受体 

分 类 号：R739.65[医药卫生—肿瘤]