非小细胞肺癌中垂体瘤转化基因的表达及临床意义  

Association of PTTG Expression with Biological Behaviors of Non-small Cell Lung Cancer

作　　者：王丽芳[1] 郜红利[1] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000

出　　处：《华西医学》2009年第11期2938-2940,共3页West China Medical Journal

摘　　要：目的:研究垂体瘤转化基因在非小细胞肺癌组织、肺良性病变组织和正常支气管黏膜上皮组织中的表达,初步探讨其与非小细胞肺癌发生发展,侵袭转移的关系。方法:(1)用SP免疫组化法检测76例临床石蜡组织标本(44例非小细胞肺癌、20例肺良性病变组织和12例正常支气管黏膜上皮组织)中的PTTG蛋白的表达。(2)用RTPCR法分析PTTG mRNA在不同性质肺组织中的表达情况。结果:(1)PTTG蛋白在不同性质肺组织中的表达差别具有明显差异;在TNM分期、淋巴结转移组间差别有统计学意义。(2)PTTG mRNA在不同性质肺组织中的表达差别具有明显差异(P<0.001);在TNM分期、淋巴结转移组间差别有统计学意义。结论:细胞肺癌的发生发展及转移有关。Objective：To investigate the association between PTTG expression and biological behaviors of human non-small cell lung cancer（NSCLC）.Methods：SP immunohistochemistry was used to detect the expression of PTTG in 76 cases of paraffin-embedded specimens（including 44 surgical specimens from NSCLC patients.20 pneumonic benign lesion and 19 normal bronchial epithelium）.Real-time RT-PCR was performed to detect PTTGm RNA expression in 44 cases of fresh carcinoma and corresponding adjacent normal mucosa specimens.Results：The expression levels of PTTG was significantly different between normal mucosa and adenoma tissues.There were statistical relationships between their expressions and TNM stage,lymphnode metastasis.Conclusion：PTTG may play an important role in carcinogenesis、development and metastasis of human non-small cell lung cancer.

关 键 词：非小细胞肺癌 垂体瘤转化基因 逆转录聚合酶链反应 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]