己酮可可碱对香烟烟雾提取物激活大鼠肺泡巨噬细胞IP-10表达的抑制作用  

Pentoxifylline Inhibits Cigarette Smoke Extract-induced IP-10 Expression in Rat Alveolar Macrophages

作　　者：汪铮[1] 张劲农[1,2] 胡小飞[1] 彭梅君[1] 王小溶[1] 赵婷婷[1] 向敏[1,2] 付薇[1,2] 

机构地区：[1]武汉协和医院呼吸病研究所,湖北武汉430022 [2]武汉协和医院呼吸内科

出　　处：《医学信息（医药版）》2009年第8期1-3,共3页

摘　　要：【摘要】目的：探讨己酮可可碱（PTX）对香烟提取物激活原代培养大鼠肺泡巨噬细胞IP-10表达的作用。方法：肺泡灌洗法收集Wistar大鼠肺泡巨噬细胞，贴壁纯化培养，制备香烟烟雾提取物（CSE），分别以1mol／L、10mol／L、100mol／L和1000mol／LPTX加入含5％CSE的培养体系中，干预24小时后ELISA法检测上清中IP-10含量。RT—PCR．法检测细胞IP-10mRNA表达。结果：1、10、100和1000mol／LPTX干预后，培养上清中IP-10的含量分别为15．82±0．43pg／ml、12．41±0．36pg／ml、7．92±0．67pg／ml、5．13±O．31pg／ml，IP-10mR．NA的表达强度分别为0．241±0．056、0．432±0．034、0．854±0．094、0．772±0．055。结论：PTX干预能剂量依赖性地抑制香烟烟雾诱导的大鼠肺泡巨噬细胞IP-10表达，该作用可能是PTX拮抗肺内炎症激活和抑制慢性吸烟肺气肿形成的机制之-。Objective To investigate the effect of pentoxifylline （PTX） on cigarette smoke extract （CSE）-induced IP-10 production in rat alveolar macrophages. Methods PTX of different concentrations and CSE were added into rat alveolar macrophage primary cultures （5% VN each）. 24 hours later, the culture samples were collected for determination of IP- 10 concentration and IP-10 mRNA expression level. Results The level of IP-10 was 15.82± 0.43 pg/ml, 12.41±0.36 pg/ml, 7.92± 0.67 pg/ml and 5.13 ± 0.31 pg/ml, and the relative densometry of IP- 10 mRNA was 0.241 ± 0.056, 0.432±0.034, 0.854±0.094, 0.772± 0.055 after CSE and PTX exposure, respectively. Conclusion PTX inhibits CSE-induced IP-10 expression in a dose-dependent manner, which may serve as one of the mechanisms involved in its preventive effect on CSE-induced lung inflammation.

关 键 词：己酮可可碱 肺泡巨噬细胞 IP-10 肺气肿 

分 类 号：R-332[医药卫生]