升结肠支撑管腹膜炎动物模型的建立  

The changes of intestinal permeability in the mouse model of colon ascendens stent peritonitis

作　　者：杨方亮[1] 王新波[1] 王思珍[1] 丁威威[1] 范欣鑫[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2010年第3期160-162,共3页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金(30830098)

摘　　要：目的:建立弥漫性腹膜炎动物模型,并观察不同程度弥漫性腹膜炎对肠黏膜通透性的影响。方法:采用升结肠支撑管腹膜炎模型(CASP)诱发弥漫性腹膜炎。将小鼠随机分为对照组、假手术组、和支撑管1.3mm组和1.7mm组。观察术后72h各组的生存率。并于术后18h比较肠黏膜通透性的变化。结果:升结肠支撑管内径越大,造成弥漫性腹膜炎小鼠死亡率越高。1.3mm组和1.7mm组术后72h累积生存率分别为75%和44%。1.7mm组肠道通透性高于1.3mm组。结论:CASP模型较好地模拟了急性弥漫性腹膜炎。当支撑管内径为1.7mm时,可模拟重度急性弥漫性腹膜炎,而当支撑管内径为1.3mm时则可模拟轻度急性弥漫性腹膜炎。Objective：To establish a mouse model of diffuse peritonitis and to investigate the changes of the intestinal permeability in mouse with diffuse peritonitis of varying severity.Methods：In the model of colon ascendens stent peritonitis,2 groups of CASP were established depending on the stent diameter （ 1.3mm group and 1.7mm group）.Survival rate was analyzed in the groups.The intestinal permeability was measured by detecting the mucosal-to-serosal clearance of FD4.Results：Survival rate after CASP procedure correlated strongly with the stent diameter.The intestinal mucosal permeability was significantly elevated in the model of CASP 18 h after operation,especially in the 1.7mm group.Conclusion：The CASP model closely mimics the diffuse peritonitis,in which the diameter of stents correlated with the severity of peritonitis.If it reached 1.7mm in diameter,severe acute peritonitis could be developed.when in the diameter was 1.3mm,only mild peritonitis could be developed.

关 键 词：升结肠支撑管腹膜炎模型 肠黏膜屏障 肠黏膜通透性 

分 类 号：R-332[医药卫生]