胶质瘤中p14^(ARF)甲基化分析及其与p53表达的相关性研究  

Methylation status of p14^(ARF) gene and its correlation with p53 protein expression in gliomas

作　　者：乔建兵[1] 何杰[2] 朱海清[3] 

机构地区：[1]东南大学附属中大医院肿瘤科,南京210009 [2]苏州大学附属第四医院病理科,无锡214062 [3]南京医科大学附属脑科医院病理科,南京210029

出　　处：《肿瘤》2010年第5期410-413,共4页Tumor

摘　　要：目的:探讨p14ARF甲基化与胶质瘤发生和预后的关系,及其与突变型p53(mutant type p53,mtp53)蛋白表达的相关性。方法:采用甲基化特异性PCR(methylation specific polymerase chain reaction,MSP)法检测33例胶质瘤和12例正常脑组织中p14ARF的甲基化状况。免疫组织化学法检测58例胶质瘤和12例正常脑组织中p14ARF和mtp53蛋白的表达。结果:胶质瘤与正常脑组织中p14ARF甲基化率分别为39.4%(13/33)和0(0/12),2组间差异有统计学意义(P<0.01)。低级别组胶质瘤甲基化率(6/15)与高级别组(7/18)间差异无统计学意义(P>0.05)。p14ARF甲基化情况与患者预后无明显相关性。mtp53蛋白在胶质瘤和正常脑组织中的阳性率分别为56.9%(33/58)和8.3%(1/12),2组间比较,差异有统计学意义(P<0.01),其表达在肿瘤高级别组中明显高于低级别组(P<0.05)。胶质瘤中mtp53蛋白表达与p14ARF甲基化呈负相关(P<0.05)。结论:p14ARF甲基化与胶质瘤发生密切相关,为胶质瘤发生的早期事件,检测p14ARF甲基化情况可作为胶质瘤早期诊断的分子生物学指标。Objective：To explore the relationship between methylation status of p14^ARF gene and tumorigenesis and prognosis of human glioma and the correlation of methylation of p14ARF gene with the expression of mutated type p53（mtp53） protein.Methods：Methylation specific polymerase chain reaction（MSP） was used to determine the methylation status of the promotor of p14^ARF gene in 33 gliomas and 12 normal brain tissues.Immunohistochemistry（IHC） method was used to detect the expression of p14^ARF and mtp53 protein in 58 cases of glioma and 12 cases of normal brain tissues.Results：The positive methylation rate of p14ARF was 39.4%（13/33） in glioma and 0（0/12） in normal brain tissues.There was a statistical difference between them（P〈0.01）.No significant difference was observed in the methylation rates of p14ARF gene between weakly and highly differentiated glioma（6/15 vs 7/18,P〉0.05）.No statistical correlation was observed between the methylation status of p14^ARF promoter and the prognosis of the patients.The positive rates of mtp53 protein in glioma and normal brain tissues were 56.9%（33/58） and 8.3%（1/12）,respectively.There was a significant difference between them（P〈0.01）.The expression of mtp53 protein was markedly higher in high-grade glioma than that in low-grade glioma（P〈0.05）.The expression of mutp53 protein and the methylation of p14^ARF gene in glioma showed a significantly negative correlation（P〈0.05）.Conclusion：Methylation of p14ARF gene was closely associated with glioma carcinogenesis.It is an early event during glioma occurrence and may be used as a molecular index for early diagnosis of glioma.

关 键 词：神经胶质瘤 甲基化 免疫组织化学 P14ARF基因 P53蛋白 

分 类 号：R739.41[医药卫生—肿瘤]