Livin和脆性组氨酸三联体的表达在确定乳腺癌保乳手术切缘及术中放疗安全靶区中的意义  

The role of expressions of Livin and fragile histidine triad in defining the cutting edge in breast-conserving surgery and the safe area of targeted intraoperative radiotherapy

作　　者：刘慧敏[1] 周士福[1] 时伟锋[1] 孟东[1] 金留根[1] 宋明旭[2] 高玮红[3] 吴玉玉[3] 

机构地区：[1]苏州大学附属第四人民医院肿瘤外科,无锡214062 [2]苏州大学附属第四人民医院中心实验室,无锡214062 [3]苏州大学附属第四人民医院病理科,无锡214062

出　　处：《肿瘤》2010年第5期419-422,共4页Tumor

摘　　要：目的:探讨凋亡抑制蛋白Livin和脆性组氨酸三联体(fragile histidine triad,FHIT)作为乳腺癌保乳手术中手术切缘和放疗安全靶区界定分子指标的可行性。方法:分别采用免疫组织化学法和RT-PCR法检测30例乳腺癌及距离肿瘤边缘1、2和3 cm处癌旁组织中Livin和FHIT蛋白及其mRNA的表达,并与HE染色病理切片进行比对。结果:Livin蛋白及其mRNA在乳腺癌组织和癌旁组织(距离肿瘤边缘1、2和3 cm)中的表达率分别为66.67%、20.83%、2.50%和0.00%,以及73.30%、19.17%、3.33%和0.00%;FHIT蛋白及其mRNA在乳腺癌组织及其癌旁组织(距离肿瘤边缘1、2和3 cm)中的表达率分别为46.67%,76.67%,82.50%和86.67%,以及53.33%、73.33%、85.00%和87.50%。Livin mRNA与FHITmRNA的表达呈负相关(r=-0.346,P<0.01)。距离肿瘤边缘1、2和3 cm处癌旁组织中Livin和FHIT的表达差异均有统计学意义(P<0.05)。结论:Livin和FHIT在乳腺癌及其不同距离癌旁组织中的表达存在差异,距离肿瘤边缘1 cm以外的范围是比较合理的术中电子线照射区域。Objective：To investigate whether apoptosis suppressor protein Livin and fragile histidine triad（FHIT） could be used as the functional molecules in defining the cutting edge in breast-conserving surgery and the safe area of targeted intraoperative radiotherapy.Methods：Immunohistochemistry and RT-PCR were performed to detect the protein and mRNA expressions of Livin and FHIT in 30 cases of breast cancer tissues and adjacent tissues 1 cm,2 cm and 3 cm distant from the margin of tumor bodies.The expressions of Livin and FHIT proteins were compared with the HE staining results.Results：The positive rates of Livin protein were 66.67%,20.83%,2.50% and 0.00% in breast cancer and adjacent tissues 1 cm,2 cm,3 cm distant from tumor bodies,respectively.The positive rates of Livin mRNA were 73.30%,19.17%,3.33%,and 0.00%,respectively.The positive rates were 46.67%,76.67%,82.50%,and 86.67% for FHIT protein and 53.33%,73.33%,85.00% and 87.50% for FHIT mRNA.The expressions of Livin mRNA and FHIT mRNA had negative correlation（r=-0.346,P〈0.01）.The differences in Livin and FHIT expressions were statistically significant between different locations（P 〈0.05）.Conclusion：The expressions of Livin and FHIT were significantly different in breast cancer and para-cancerous tissues with varying distance to the tumor margins.The adjacent tissues with distance of more than 1 cm to the margin of tumors were suitable for intra-operative electron irradiation.

关 键 词：乳腺肿瘤 术中放疗 LIVIN 脆性组氨酸三联体 

分 类 号：R737.9[医药卫生—肿瘤]