人骨形态发生蛋白2,3,6,12对大鼠骨肉瘤细胞株UMR106的作用  

Effect of human bone morphogenetic protein 2,3,6,and 12 on osteosarcoma cell line UMR106

作　　者：李星星[1] 何焕玲[1] 吴丽美[1] 马闻[1] 陈英华[1] 卫佳[1] 苗静琨[1] 周兰[1] 

机构地区：[1]重庆医科大学生物医学工程系,重庆市生物医学工程学重点实验室,重庆市超声医学工程重点实验室-省部共建国家重点实验室培育基地,重庆400016

出　　处：《中南大学学报（医学版）》2010年第5期464-469,共6页Journal of Central South University ：Medical Science

基　　金：重庆市自然科学基金(CSCT2005BB5320)~~

摘　　要：目的:研究人骨形态发生蛋白(human bone morphogenetic proteins,hBMP)对大鼠骨肉瘤细胞株UMR106的作用。方法:用hBMP2,3,6,12重组腺病毒(AdBMP2,3,6,12)干预大鼠骨肉瘤细胞株UMR106,利用台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶(AO/EB)荧光双染法、Transwell小室和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化。结果:与空白对照组和实验对照组比较,AdBMPs的干预致骨肉瘤细胞株细胞存活率降低,并呈一定的时间依赖性;凋亡率均随时间延长而增加,并且TUNEL和AO/EB两种检测方法的结果一致;3个检测时间点的细胞穿膜数均明显减低;碱性磷酸酶(alkaline phosphatase,ALP)活性在干预第3天开始逐渐增加,至第9天仍可观测到,以上差异均有统计学意义(P<0.01)。结论:以腺病毒介导基因转入的作用方式,hBMP2,3,6,12可以抑制大鼠骨肉瘤细胞株UMR106的增殖和迁移,并诱导其凋亡和向成骨细胞分化。Objective To investigate the effect of human bone morphogenetic protein（hBMPs） 2/3/6 and 12 on osteosarcoma cell UMR106.Methods Adenovirus-BMP2/3/6 and 12（AdBMP2/3/6 and12） were used to treat the cell line.Their proliferation,apoptosis,and transmigration were detected by Trypan blue exclusion test,TdT-mediated biotinylated-dUTP nick end labling（TUNEL）,acridine orange-ethidium bromide（AO/EB） double fluorescent dye staining,and transwell-room test,respectively.The alkaline phosphatase（ALP） activity was detected to reflect the differentiation of tumors.Results Compared with the control groups,the cell survival rate of the experimental groups treated with AdBMP2/3/6 and 12 showed a significant time-dependent decrease（P〈0.01）.The apoptosis indexes were increased significantly（P〈0.01） and the results from TUNEL and AO/EB method were consistent.The cell numbers of transmembrane significantly decreased at 24,48,and 72 h（P〈0.01）.AdBMP2/3/6 and 12 treatment enhanced the activity of ALP activity from day 3 and this effect might still be observed up to day 9 of the treatment（P〈0.01）.Conclusion hBMPs2/3/6 and 12 can inhibit the proliferation and transmigration,and induce their apoptosis and differentiation in osteosarcoma cell line UMR106.

关 键 词：骨形态发生蛋白 细胞增殖 细胞凋亡 细胞迁移 碱性磷酸酶活性 

分 类 号：R738[医药卫生—肿瘤]