近交系小鼠牙胚同种异体移植至口腔黏膜下成牙能力的研究  

Dentification ability of inbred strain mice tooth germs homologically transplanted into oral submucosa

作　　者：李恒[1] 阙国鹰[1] 张磊[2] 

机构地区：[1]中南大学湘雅医院口腔科,长沙410008 [2]中南大学湘雅口腔医学院,长沙410008

出　　处：《中南大学学报（医学版）》2010年第5期524-528,共5页Journal of Central South University ：Medical Science

基　　金：中南大学大学生创新性试验计划(YA07053)~~

摘　　要：目的:观察近交系BALB/C小鼠牙胚移植至口腔黏膜下的成牙能力,为组织工程化牙齿的培养寻找有利的体内环境。方法:将出生后4 d BALB/C小鼠的下颌第一磨牙牙胚植入BALB/C成年雄鼠的口腔黏膜下,分别于植入后1,2,3,6周取材,HE染色,观察牙胚发育的形态学变化。结果:牙胚在口腔黏膜下生长良好,釉质和牙髓牙本质复合体继续发育,牙本质小管结构清晰可见。结论:BALB/C小鼠牙胚移植至BALB/C成年雄鼠口腔黏膜下能够进一步发育矿化,可为组织工程化牙齿的构建提供简便合适的发育环境。Objective To establish a suitable environment for the bioengineered teeth in vivo by observing the dentification ability of BALB/C mice tooth germs homologically implanted into the oral submucosa.Methods The first molar tooth germs of BALB/C mice 4 days after birth were transplanted into the oral submucosa of BALB/C male mice,and then recycled for regular histological observation after 1,2,3,and 6 week transplantation.Results The tooth germs in the oral submucosa grew well with continuing developing enamelum and pulpodentinal complex,and the dentinal tubules were clear.Conclusion The environment of the BALB/C male mice oral submucosa is favorable for the growth of tooth germs in inbred strain BALB/C mice,and it can provide a new environment for the development of bioengineered teeth in vivo.

关 键 词：近交系小鼠 牙胚 移植 组织工程 

分 类 号：R783[医药卫生—口腔医学]