非小细胞肺癌PHDsmRNA表达及与Bax和Bcl-2的关系  

Relationship between expression of PHDsmRNA and Bax and Bcl-2 in non-small cell lung cancer

作　　者：陈苏峰[1] 张杰[2] 朱晓丽[3] 姚仕华[1] 张军华[1] 陈海泉[2] 

机构地区：[1]上海交通大学第六人民医院心胸外科,上海200233 [2]复旦大学肿瘤医院胸外科,上海200032 [3]复旦大学肿瘤医院病理科,上海200032

出　　处：《上海交通大学学报（医学版）》2010年第5期570-573,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究非小细胞肺癌中脯氨酰羟化酶基因(PHDs)mRNA的表达及其与凋亡相关蛋白Bax和Bcl-2表达的关系方法分别采用Real-time PCR技术和免疫组织化学法,检测56例非小细胞肺癌患者肿瘤组织和癌旁正常组织PHD1、2、3基因的表达及癌组织Bax、Bcl-2的表达。结果 56例非小细胞肺癌患者中,肺癌组织PHDsmRNA表达均高于癌旁正常组织,差异均有统计学意义(P<0.05)。Bax和Bcl-2阳性表达病例分别占33.9%(19/56)和32.1%(18/56)。PHD3 mRNA高表达和低表达癌组织中Bcl-2阳性表达率分别为19.5%和66.7%,差异有统计学意义(P<0.05)。结论非小细胞肺癌组织中PHDs mRNA表达增强。PHD3 mRNA表达上调可能与细胞凋亡有关。Objective To investigate the expression of prolyl hydroxylase domain enzymes genes（PHDs mRNA）,and explore its relationship with expression of apoptosis-related protein Bax and Bcl-2 in non-small cell lung cancer.MethodsThe expression of PHD1,2,3 mRNA and Bax and Bcl-2 in cancer tissues and para-cancerous normal tissues in 56 cases of non-small cell lung cancer was detected by Real-time PCR and immunohistochemical staining,respectively.ResultsThe expression of PHDs mRNA in cancer tissues was significantly higher than that in para-cancerous normal tissues（P0.05）.Patients with positive expression of Bax and Bcl-2 accounted for 33.9%（19/56）and 32.1%（18/56）,respectively.The positive expression rates of Bcl-2 in cancer tissues with high expression and low expression of PHD3 mRNA were 19.5% and 66.7%,respectively.Conclusion The expression of PHDs mRNA is increased in non-small cell lung cancer tissues,and the increased expression of PHD3 mRNA may be associated with cell apoptosis.

关 键 词：脯氨酰羟化酶 非小细胞肺癌 凋亡 BCL-2 BAX 

分 类 号：R734.2[医药卫生—肿瘤]