淫羊藿苷对大鼠间充质干细胞骨向分化过程中转化生长因子β_1、骨形态发生蛋白2表达的影响  

Effect of icariin on the expression of transforming growth factor-beta 1 and bone morphogenetic protein-2 in the process of mesenchymal stem cells differentiation into osteoblasts

作　　者：杨丽[1] 张荣华[1] 朱晓峰[2] 蔡宇[1] 黄丰[3] 

机构地区：[1]暨南大学药学院中药学教研室,广东省广州市510632 [2]暨南大学附属第一医院中医科,广东省广州市510632 [3]暨南大学药学院药理学教研室,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2010年第19期3518-3522,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30472274);广东省自然科学基金重点资助项目(04105837);广东省社会发展计划重点引导项目(2004B33001004)~~

摘　　要：背景:淫羊藿苷作为补肾中药淫羊藿的主要有效成分,能够有效促进间充质干细胞向成骨细胞分化。目的:观察淫羊藿苷在诱导间充质干细胞骨向分化过程中对转化生长因子β1、骨形态发生蛋白2表达的影响,阐释其可能的诱导机制。方法:运用全骨髓贴壁法分离、纯化大鼠骨髓间充质干细胞;以20mg/L淫羊藿苷作为间充质干细胞药物干预浓度,根据干预条件的不同将实验分为:空白组、经典组(加经典成骨诱导剂)、淫羊藿苷组、淫羊藿苷+经组;ELISA法检测各组转化生长因子β1、骨形态发生蛋白2的表达。结果与结论:经典组、淫羊藿苷组、淫羊藿苷+经组转化生长因子β1、骨形态发生蛋白2值均高于空白组(P<0.05);各组第7天的转化生长因子β1值高于14,21d(P<0.01),各组不同诱导时间骨形态发生蛋白2值之间相比差异无显著性意义(P>0.05)。提示上调转化生长因子β1、骨形态发生蛋白2的表达量可能是淫羊藿苷诱导各组促进间充质干细胞骨向分化的作用机制之一。BACKGROUND：As the main effective ingredient of epimedium,icariin（ICA） can promote the differentiation of mesenchymal stem cells（MSCs） into osteoblasts.OBJECTIVE：To observe effects of ICA on the expression of transforming growth factor-β1（TGF-β1） and bone morphogenetic protein-2（BMP-2） in the process of mesenchymal stem cells differentiation into osteoblasts,and to explain the inducing mechanisms.METHODS：MSCs were isolated and purified by whole marrow adherent method.20 μg/mL ICA was used to be the intervention concentration;according to the different induced conditions,MSCs were divided into 4 groups：blank group,classic group（induced by the classic osteoblast-induced system）,ICA group（induced by 20 μg/mL ICA） and classic＋ICA group（induced by the combination of classic osteoblast-induced system and 20 μg/mL ICA）.ELISA was used to detect the expression of TGF-β1 and BMP-2 of each induced group in the progress of MSCs differenting into osteoblasts.RESULTS AND CONCLUSION：The expression of TGF-β1 and BMP-2 of the classic group,ICA group and classic＋ICA group were all higher than that of the blank group（P 0.05）;the expression of TGF-β1 of each group was higher at 7 days than that at 14 and 21 days（P 0.01）;however,the expression of BMP-2 of each group at 7,14 and 21 days had no statistical difference（P 0.05）.The results revealed that upregulation of the expression of TGF-β1 and BMP-2 in the ICA-induced groups may be the mechanisms on improving the differentiation of MSCs into osteoblasts.

关 键 词：淫羊藿苷 骨髓间充质干细胞 大鼠 转化生长因子Β1 骨形态发生蛋白2 

分 类 号：R394.2[医药卫生—医学遗传学]