实验性糖尿病牙周炎牙周膜中成纤维细胞凋亡的初步研究  

Fibroblasts apoptosis in the periodontal ligament in experimental diabetic periodontitis in rats

作　　者：付永伟[1] 和红兵[2] 欧炯光[2] 

机构地区：[1]连云港市第一人民医院口腔科,222002 [2]昆明医学院附属口腔医院牙周病科

出　　处：《实用口腔医学杂志》2010年第3期310-314,共5页Journal of Practical Stomatology

摘　　要：目的:初步探讨糖尿病牙周炎条件下牙周膜中成纤维细胞的凋亡情况。方法:选用6周雄性SD大鼠62只,随机分为糖尿病牙周炎组(DP)、牙周炎组(P)和正常对照组(N)。采用一次性腹腔注射STZ的方法诱导大鼠糖尿病模型,采用丝线结扎联合口内接种细菌的方法建立牙周炎模型。动物分别于丝线结扎后3周和6周分批处死,进行HE染色和原位细胞凋亡检测。观察指标包括:组织病理学比较,牙周膜中成纤维细胞计数,牙周膜中成纤维细胞凋亡百分率。资料采用单因素方差分析统计学处理。结果:丝线结扎后3周和6周,与N组比较,P组和DP组单位面积牙周膜中成纤维细胞数减少,与P组比较,DP组单位面积牙周膜中成纤维细胞数亦显著减少(P<0.05)。牙周膜中成纤维细胞凋亡百分率DP组>P组>N组,组间两两比较均有统计学意义(P<0.05)。在丝线结扎后3周和6周,DP组牙周膜中成纤维细胞凋亡百分率均达到P组的2倍左右。结论:糖尿病可加强牙周炎条件下牙周膜中成纤维细胞的凋亡,降低这些细胞的数量。Objective：To observe fibroblasts apoptosis in the periodontal ligament in experimental diabetic periodontitis.Methods：Sixty-two six-week-old male SD rats were randomly divided into three groups：diabetic periodontitis group（DP）,periodontitis group（P）and normal control group（N）.Diabetic rats were induced by intraperitoneal injection of streptozototocin（STZ）.Periodontitis was initiated by ligating floss around maxillary second molars and oral inoculation with periodontal pathogen Porphyromonas gingivalis.Three or six weeks later,the animals were sacrificed and the specimens were prepared for histological analysis.Assessment was carried out using histological approaches.Histological examination was used to detect fibroblasts numbers in the periodontal ligament,the percentage of apoptotic fibroblasts in the periodontal ligament.Statistical significance was determined by one-way ANOVA with significance set at P0.05.Results：Intergroup comparisons demonstrated that periodontitis and diabetic periodontitis might reduce the number of fibroblasts in the periodontal ligament.Moreover,the number of fibroblasts in the periodontal ligament in group DP was also decreased compared with group P（P0.05）.Apoptosis of fibroblasts was also increased in periodontitis and diabetic periodontitis.The percentage of apoptotic fibroblasts in the periodontal ligament was approximately 2-fold higher in group DP compared with group P.Conclusion：The present study clearly demonstrates that diabetes can increase the severity of periodontitis.Diabetes can increase the apoptosis of fibroblasts in the periodontal ligament.

关 键 词：牙周炎 糖尿病 成纤维细胞 凋亡 

分 类 号：R781.42[医药卫生—口腔医学] R587.1[医药卫生—临床医学]