大鼠破裂型椎间盘突出模型的建立及突出物重吸收机制的研究  

A study of a rat lumbar disc herniation model and the mechanism spontaneous of resorption

作　　者：刘锦涛[1] 姜宏[1] 王拥军[2] 徐坤林[1] 张志刚[1] 李红卫[1] 

机构地区：[1]苏州市中医医院骨科,江苏苏州215003 [2]上海中医药大学脊柱病研究所

出　　处：《中国骨伤》2010年第5期370-372,共3页China Journal of Orthopaedics and Traumatology

摘　　要：目的:设计一种新的腰椎间盘突出重吸收模型。方法:取20只体重(300±20)g的3月龄远交群(SD)雄性大鼠,依据随机数字表随机分成实验组和对照组。实验组经手术切除尾椎椎间盘,埋植在硬膜外;动物30d后处死,取埋植髓核组织,进行HE染色,流式细胞仪以及免疫组化检测。对照组,将线团埋植在背部肌肉内,30d后处死作实验对照。结果:实验组30d后的TNFɑ、VEGF免疫组化染色呈阳性。实验组的Th、B细胞数量与对照组比较差异有统计学意义。结论:大鼠破裂型椎间盘突出模型能够很好的揭示破裂型椎间盘突出后的重吸收过程,为进一步研究椎间盘突出后的重吸收提供一种新的动物模型。Objective：To establish a new animal model of resorption of lumbar disc herniation. Methods：Twenty 3-month-old Sprague Dawley（SD） male rats were randomly divided into experimental group and control group. The caudal discs of the experimental rats were surgically removed and were implanted in the epidural. The animals were killed at 30 days,and the implanted nucleus pulposus were took out for HE staining,flow cytometry and immunohistochemistry. In the control group,coils were implanted in the back muscles,and the animals were killed at 30 days after the operation for the above test. Results：In the experimental group,immunohistochemical staining of TNF-ɑ,VEGF were positive at 30 days. The Th cells and B cells in the experimental group were more than that in the control group with statistically significant differences. Conclusion：The model can well reveal the re-absorption processthe of the ruptured disc,and provide a new re-absorption disc animal model for the further study.

关 键 词：椎间盘移位 吸收 模型 动物 大鼠 

分 类 号：R[医药卫生]