伽玛刀低剂量照射对致疒间大鼠海马中氨基酸递质的影响  

Effect of low-dose Gamma Knife radiation on amino acid transmitters in the hippocampus of epileptic rats

作　　者：武登峰[1] 梁军潮[2] 关慧[3] 曾凡猛[2] 伍犹梁[2] 张聿浩[2] 刘德平[2] 王伟民[2] 

机构地区：[1]南方医科大学 [2]广州军区广州总医院神经外科,510010 [3]广州军区广州总医院药学部,510010

出　　处：《中国微侵袭神经外科杂志》2010年第5期228-230,共3页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的观察伽玛刀低剂量照射对致疒间大鼠海马中谷氨酸(Glu)、γ-氨基丁酸(GABA)的影响,探讨伽玛刀治疗癫疒间的作用机制。方法将50只锂-匹罗卡品致疒间大鼠随机分为照射组和非照射组,各25只,另取正常大鼠25只(腹腔注射生理盐水)作为对照组。照射组大鼠进行伽玛刀低剂量照射(周边剂量20Gy),对照组和非照射组大鼠不进行伽玛刀照射。用高效液相色谱-质谱-质谱(HPLC-MS-MS)分析法检测各组大鼠海马Glu、GABA的含量。结果伽玛刀照射2周后,照射组和非照射组大鼠海马中Glu含量均高于对照组,照射组GLu含量低于非照射组,差异具有统计学意义(P<0.05);照射组和非照射组GABA含量均低于对照组,照射组GABA含量高于非照射组,差异具有统计学意义(P<0.05)。结论伽玛刀低剂量照射通过调节海马中Glu与GABA的平衡,以达到抗癫疒间的作用。Objective To explore the mechanism of Gamma Knife radiosurgery for epilepsy by studying the effect of low-dose Gamma Knife radiosurgery on glutamate （Glu） and γ-aminobutyric acid （GABA） in the hippocampus of epileptic rats. Methods Fifty Li-pilocarpine-induced epileptic rats were randomly divided equally into radiation group and non-radiation group. Additional twenty-five normal rats were treated with normal sodium as the control group. The radiation group rats were treated by Gamma Knife radiation at a marginal dose of 20 Gy and no radiation was performed in non-radiation and control groups. Glu and GABA contents in the hippocampus of epileptic rats were detected by high performance liquid chromatography-mass spectrometry-mass spectrometry （HPLC-MS-MS） in all groups. Results After two weeks of radiation,the contents of Glu in the hippocampus of the rats in non-radiation and radiation groups were significantly higher than control group,and that of radiation group was significantly lower than non-irradiation group （P0.05）. The contents of GABA in the hippocampus of the rats in non-irradiated and irradiated group were significantly lower than control group,and that of radiation group was significantly higher than non-irradiated group （P0.05）. Conclusions Low-dose Gamma Knife irradiation can counteract epilepsy through the regulation of the balance of Glu and GABA in the hippocampus.

关 键 词：癫 放射外科手术 海马 谷氨酸 Γ-氨基丁酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学]