基于体视学参数的肺癌组织芯片可靠性探讨  

Comparison of the reliability of stereological measurements of lung cancer tissue based on microarray and routine paraffin sections

作　　者：赵云飞[1,2] 申洪[1,2] 

机构地区：[1]南方医院病理科 [2]南方医科大学基础医学院病理学教研室,广州510515

出　　处：《中国体视学与图像分析》2010年第1期84-88,共5页Chinese Journal of Stereology and Image Analysis

摘　　要：目的比较石蜡组织芯片和相应常规组织切片正常成人肺泡Ⅱ型上皮细胞、人胚胎肺泡上皮细胞和肺癌细胞的细胞核体视学参数,探讨组织芯片代表性的可靠程度。方法收集肺癌手术切除标本:鳞癌52例、腺癌38例、大细胞癌10例、小细胞癌4例、正常肺组织20例和胚胎肺组织20例。采用组织微阵列技术构建760点阵石蜡组织芯片。用Image-Pro图像分析软件测试胞核及细胞,按体视学公式计算以细胞为参照空间时肺癌细胞核的体积密度(Vvn)、表面积密度(Svn)、表面积与体积比(Rsv,n)、核浆比(Rnp)、平均自由程(λn)。结果石蜡组织芯片和相应常规组织切片细胞核的体视学参数基本类同,两者比较差异无显著性,P值均>0.05。结论组织芯片和相应常规组织切片细胞核的体视学参数基本类同,组织芯片具有较好的可靠性。Objective To compare the measurements of nuclear stereological parameters of normal adult alveolar type Ⅱ epithelial cells,human embryonic lung cells and lung cancer cells in conventional paraffin-embedded tissue sections and microarrays a,and to explore the reliability of stereological quantification based on tissue microarray.Methods Surgical resection of lung cancer specimens： 52 cases of squamous cell carcinoma,38 cases of adenocarcinoma,10 cases of large cell carcinoma,4 cases of small cell carcinoma,20 cases of normal lung tissue and 20 cases of embryonic lung tissue.The TMA technology was used to construct a paraffin tissue microarray containing 760 tissue cores.Using Image-Pro image analysis system to test cells and their nuclear-related stereological parameters：Vvn,Svn,Rsv,Rnp and λn.The reference space of nucleus was according to the cancer cells and that of nuclei was according to the nucleus.Results The values of stereological parameters of the nuclei measured on paraffin tissue microarrays and corresponding routine histological sections were basically similar,with statistically non-significant differences between them,P0.05.Conclusion The results of this study demonstrate that stereological measurement of cell nuclei based on both tissue microarrays and the corresponding routine histological sections is reliable and basically similar,but the use of tissue microarray is feasible and more efficient.

关 键 词：肺癌 组织芯片 体视学 可靠性 

分 类 号：R734.2[医药卫生—肿瘤]