95%水胺硫磷慢性毒性和致癌性研究  

Chronic Toxicity and Carcinogenicity of 95% Isocarbophos in Rats

作　　者：张晓鹏[1] 张馨[1] 王伟[1] 崔文明[1] 刘兆平[1] 向钱[1] 贾旭东[1] 宋雁[1] 张文众[1] 李宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2010年第3期229-232,共4页Chinese Journal of Food Hygiene

摘　　要：目的研究95%水胺硫磷的慢性毒性和致癌性,提出其未观察到有害作用水平。方法480只断乳SD大鼠按体重随机分成4组,每组120只,雌雄各半。4组大鼠喂饲含有不同浓度水胺硫磷的块料,对照组、低剂量组、中剂量组和高剂量组的剂量分别是0、0.6、6和30mg/kg饲料,试验期2年。观察大鼠日常表现,检测体重、进食量、血常规、凝血时间、血生化、胆碱酯酶、尿常规等指标并进行大体和病理组织学检查,计算食物利用率、脏器系数和肿瘤发生率。结果试验第6、18、24个月,高剂量组雌性大鼠胆碱酯酶活性显著低于对照组(P<0.01);试验第6、12、18和24个月,中、高剂量组雄性大鼠胆碱酯酶活性显著低于对照组(P<0.01)。各组大鼠的其他指标,包括肿瘤发生率差异均无统计学意义。结论根据胆碱酯酶的检测结果确定水胺硫磷的未观察到有害作用水平:雌性为0.468mg/(kgBW·d)(中剂量),雄性为0.037mg/(kgBW·d)(低剂量)。未见致癌作用。Objective To study the chronic toxicity and carcinogenicity of 95% isocarbophos and to provide its no observed adverse effect level（NOAEL）.Method Four hundred and eighty male and female SD rats were divided into 4 groups and fed with foods containing 0,0.6,6 and 30 mg /kg isocarbophos respectively for 2 years.The daily behavior,body weight,food consumption,blood biochemistry,clotting time,cholinesterase activity,gross anatomy and histopathology,and the incidence of tumors were examined and analyzed.Results Activities of cholinesterase were significant decreased in some females（at the 6,18,24 month of the 30 mg /kg group） and males（at the 6,12,18,24 month of the 6 mg /kg and 30 mg /kg groups）.There were no significant changes at any level of isocarbophos on any other parameters including the incidence of tumor in any organ or tissue.Conclusion The NOAEL of isocarbophos were 0.486 and 0.037 mg /（kg BW·d） for female and male respectively,and isocarbophos is non-carcinogenic under the conditions of the study.

关 键 词：水胺硫磷 大鼠 慢性毒性 致癌性 未观察到有害作用水平 

分 类 号：R114[医药卫生—卫生毒理学]