ANIT诱导的肝内胆汁淤积大鼠肝胆系统AQP1的表达  

Expression of AQP1 in Hepatobiliary System of Rats with ANIT-induced Cholestasis

作　　者：吴一中[1] 周会新[1] 胡小宣[1] 刘展[1] 李毅妮[1] 

机构地区：[1]湖南省人民医院消化科,湖南长沙410005

出　　处：《医学临床研究》2010年第5期825-827,共3页Journal of Clinical Research

摘　　要：[目的]探讨水通道蛋白1（AQP1）在α-萘异硫氰酸（ANIT）诱导的肝内胆汁淤积大鼠肝胆系统的表达情况.[方法]将健康雄性Wistar成年大鼠16只随机分为ANIT组和对照组, 每组8只.ANIT组由10 g/L ANIT花生油溶液一次性灌胃诱导肝损伤.对照组仅灌花生油.检测两组AQP1的表达.平均光密度（OD）值.[结果]两组大鼠肝胆系统均有AQP1表达.对照组OD值为（0.38±0.08）明显高于ANIT组（0.10±0.03）（P〈0.01）.[结论]ANIT诱导的胆汁淤积大鼠肝胆系AQP1的蛋白表达下调,提示AQP1参与胆汁淤积的病理机制.[Objective]To investigate the change of aquaporinl（AQP1） expression in hepatobiliary system of rats with alpha-naphthylisothioeyanate （ANIT）-indueed cholestasis. [Methods]Sixteen healthy Wistar male adult rats were randomly divided into ANIT group and control group with 8 for each. The control group was treated with only peanut oil. The ANIT group was treated with ANIT peanut oil. A single oral dose of 150 mg/kg ANIT dissolved in peanut oil was given to rats by gavage（10g/l） to induce liver injury. Itnmunohisto- chemical method was used to detect AQP1 expression. The image of three sections of each rat was analyzed and the average gray level was determined. [Results]A single oral ANIT could induce cholestasis successfully. The expression of AQP1 protein was detected in two groups. AQP1 protein level in ANIT group was 0.10± 0.03 which was significantly lower than that（0.38±0.08） in control group（ P 〈0.01）. [Conclusion] The AQP1 expression in hepatobiliary system of rats with ANIT-induced cholestasis is significantly decreased. It suggests that AQP1 involves in the pathological mechanism of cholestasis.

关 键 词：胆汁郁积 肝内 大鼠 水孔蛋白类 

分 类 号：R575[医药卫生—消化系统]